May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
The Killing of Haemophilus Influenzae by Gatifloxacin, Levofloxacin, Ciprofloxacin, and Ofloxacin
Author Affiliations & Notes
  • P.D. Hedlin
    Microbiology & Immunology, Univ of Saskatchewan, Saskatoon, SK, Canada
  • J. Blondeau
    Microbiology & Immunology, Univ of Saskatchewan, Saskatoon, SK, Canada
  • Footnotes
    Commercial Relationships  P.D. Hedlin, Allergan F; J. Blondeau, Allergan F.
  • Footnotes
    Support  Allergan
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4264. doi:
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      P.D. Hedlin, J. Blondeau; The Killing of Haemophilus Influenzae by Gatifloxacin, Levofloxacin, Ciprofloxacin, and Ofloxacin . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4264.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: H Influenzae can be a dangerous ocular pathogen, and is prevalent in the pediatric population. The ultimate goal of antimicrobial therapy is eradication of the pathogen and clinical resolution of infection. The purpose of the current study was to compare the killing of H influenzae (HI) by 4 different fluoroquinolones at the MIC for each drug. Methods: A total of 4 clinical isolates of (HI) were used in this study. These strains were grown up in Todd–Hewitt broth to a concentration of 106–109 CFU/mL. One of the following antimicrobial agents (gatifloxacin [G], levofloxacin [L], ciprofloxacin [C], or ofloxacin [O]) was added to concentrations that were based on the MIC of the drug. The log10 reduction of viable cells was recorded at 30 minutes; and 1, 2, 4, 6, 12 and 24 hours. Results:Exposure of 106–109 CFU/mL of HI at MIC drug concentrations for 30 min–24 h to the following antimicrobial agents caused the following ranges of log reductions: G: –1.74 to 0.068; L: –2.43 to 1.34; O: –2 to 2.46; C: –2.84 to 1.58. Conclusions: The fourth–generation agent, gatifloxacin, provides a much faster and complete killing of H influenzae than do the third–generation fluoroquinolones.

Keywords: antibiotics/antifungals/antiparasitics • bacterial disease 
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