Purchase this article with an account.
R.A. Leitgeb, B. Hermann, B. Povazay, H. Sattmann, S. Michels, U. Schmidt–Erfurt, W. Drexler; Spectroscopic Analysis of the Human Retina Using Three–Dimensional Spectroscopic Ultrahigh Resolution Optical Coherence Tomography . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4273.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:To use the different spectroscopic and scattering properties of tissue for enhancing image contrast and outlining specific pathologic changes reflected by those parameters. Methods: A high speed ultrahigh resolution frequency domain optical coherence system (UHR FDOCT) has been developed that allows acquiring 25 tomograms per second with 1024x1024 pixels each. The achieved axial resolution is less than 3µm. With FDOCT one records the spectrum of the backscattered light in order to reconstruct the sample depth structure; it offers therefore direct access to spectroscopic properties of the sampled tissue. Those properties reflect the scattering and absorption properties, which are determined by chromophore (melanin, hemoglobin) concentrations as well as cellular structure. Results: UHR FDOCT images retinal structure with resolutions up to 3µm. It offers retinal tomograms that are close to the level of histology. The high speed abilities of FDOCT allow recording of three dimensional retinal volumes, which has a strong impact on the acuity of retinal diagnosis. Spectroscopic analysis of the tissue increases the dimensionality of the imaging space and provides additional information about the sampled tissue. Retinal tomograms of healthy volunteers were spectroscopically analyzed and compared to the results of different retinal pathologies. One obtaines additional information about chromophore concentrations and tissue scattering that ultimately leads to a tissue contrast enhancement. Conclusions: The high speed capabilities of FD OCT allows to record three dimensional retinal volumes. Spectroscopic analysis of the backreflected sample light gives additional information about tissue structure and composition. This help ultimately for an enhancement of tissue contrast. Moreover, changes of spectroscopic parameters could be an early indicator to retinal pathologies.
This PDF is available to Subscribers Only