May 2005
Volume 46, Issue 13
ARVO Annual Meeting Abstract  |   May 2005
Fundus Autofluorescence Imaging in Post–Mortem, Intact Human and Pig Eyes
Author Affiliations & Notes
  • A.D. Marmorstein
    Ophthalmology, University of Arizona, Tucson, AZ
  • A.M. Floyd
    Ophthalmology, University of Arizona, Tucson, AZ
  • Footnotes
    Commercial Relationships  A.D. Marmorstein, Cleveland Clinic Foundation P; A.M. Floyd, None.
  • Footnotes
    Support  NIH Grant EY014898, Philip Morris External Research Program, Research to Prevent Blindness
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4286. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A.D. Marmorstein, A.M. Floyd; Fundus Autofluorescence Imaging in Post–Mortem, Intact Human and Pig Eyes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4286.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To test the hypothesis that fundus fluorescence ratiometry might be a useful diagnostic tool for retinal disease by distinguishing autofluorescence associated with lipofuscin from autofluorescence associated with sub–RPE deposits. Methods: Human and pig eyes were imaged using a modified fundus camera after scraping of the corneal epithelium and phacoemulsification of the lens and lens capsule or after dissection of the anterior segments and vitreous. Reflected light images were captured and used as an index. Fluorescence was excited using using 405,nm, 430nm, 450nm, or 470nm light. Emissions bandwidth was varied using different combinations of barrier filters. Two emissions bandwidths were simultaneously collected. A ratiometric map of the fundus was then created using Metamorph software. After imaging the eyes were fixed and processed for paraffin embedding and stained using hematoxylin and eosin. Results: We have successfully generated both reflected light and fluorescence images from human and pig eyes. Initial data suggest that significant differences in Ratiometric comparisons indicate significant differences in emissions from the wavelengths used that may correlate with the development of sub–RPE deposits. Conclusions: This study provides the basis for continuing studies to determine whether features identified using fundus fluorescence ratiometry correspond to pathologic deposits associated with diseases such as age–related macular degeneration.

Keywords: imaging/image analysis: clinical • age-related macular degeneration • Bruch's membrane 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.