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T. Naoumidi, I. Pallikaris, I. Naoumidi; Histologic Effects of Conductive Keratoplasty (CK) on Human Corneas . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4347.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine the morphologic changes in human corneas over time following radiofrequency–based CK treatment. Methods: Lasting changes in corneal curvature following CK treatment depend on shrinkage of corneal collagen to an effective but safe depth. Six human corneas with localized peripheral keratoconus (KC) underwent CK treatment in areas free of KC changes followed by penetrating keratoplasty. Corneal buttons were examined with light and electron microscopy up to six months post–CK. Results: In samples assessed on days one and three post–CK, small areas of detachment between the basal layer of epithelial cells and Bowman’s layer were observed. At one week after the CK procedure the epithelium appeared normal. Endothelium and Descemet’s membrane showed no alterations. In all samples thermally induced misconfiguration of collagen fibers, described as "crumpled" changes of collagen, was observed reaching 75–80% of the stromal depth. The alteration had a cylindrical shape with a diameter of 120 microns. Conclusions: The conductive keratoplasty procedure produced collagen "shrinking" changes in human corneas which were observed during the follow–up of 6 months. Areas adjacent to treatment site were minimally damaged.
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