May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Donor–Derived Fibroblasts in the Pathogenesis of Lacrimal Gland Chronic Graft–Versus–Host Disease
Author Affiliations & Notes
  • Y. Ogawa
    Department of Ophthalmology,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • H. Kodama
    Department of Internal Medicine,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • K. Kameyama
    Department of Diagnostic Pathology,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • K. Yamazaki
    Department of Diagnostic Pathology,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • H. Yasuoka
    Department of Internal Medicine,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • S. Okamoto
    Department of Internal Medicine,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • K. Tsubota
    Department of Ophthalmology,
    School of Medicine Keio Univ, Shinjuku–ku, Japan
  • H. Inoko
    Department of Molecular Life Science, School of Medicine Tokai Univ, Shinjuku–ku, Japan
  • Y. Kawakami
    Institute for Advanced Medical Research, School of Medicine, Keio University, Shinjuku–ku, Japan
  • M. Kuwana
    Institute for Advanced Medical Research, School of Medicine, Keio University, Shinjuku–ku, Japan
  • Footnotes
    Commercial Relationships  Y. Ogawa, None; H. Kodama, None; K. Kameyama, None; K. Yamazaki, None; H. Yasuoka, None; S. Okamoto, None; K. Tsubota, None; H. Inoko, None; Y. Kawakami, None; M. Kuwana, None.
  • Footnotes
    Support  the Japanese Ministry of Education, Science, Sports, and Culture
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4409. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Y. Ogawa, H. Kodama, K. Kameyama, K. Yamazaki, H. Yasuoka, S. Okamoto, K. Tsubota, H. Inoko, Y. Kawakami, M. Kuwana; Donor–Derived Fibroblasts in the Pathogenesis of Lacrimal Gland Chronic Graft–Versus–Host Disease . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4409.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Excessive fibrosis is a prominent histologic feature of chronic graft–versus–host disease (GVHD) following allogeneic hematopoietic stem cell transplantation, but the underlying mechanism remains unknown. We investigated whether the fibroblasts increased at the site of pathogenic fibrosis are originated from transplanted donor cells in patients with lacrimal gland chronic GVHD. Methods: Lacrimal gland biopsies were obtained from nine patients with chronic GVHD. The male–specific sequences detected by fluorescein in situ hybridization and in situ hybridization were used as markers for the donor cells in seven female patients who had received a transplant from male donors. In addition, primary fibroblast cultures were generated from lacrimal gland biopsies and examined for mismatched genetic markers between recipients and donors. Results: In seven female patients who received a sex–mismatched transplant, fluorescein in situ hybridization for the Y–chromosome showed that 13·4–26·7% of fibroblasts accumulated in the lacrimal gland tissue were donor–derived. The male–specific mRNA was also detected in the lacrimal gland fibroblasts by in situ hybridization. The donor–origin of the fibroblasts was further confirmed by detecting the Y–chromosome sequence and the donor–specific microsatellite genetic markers in primary fibroblast cultures. Conclusions: The donor–derived fibroblasts are present in a chimeric state in the lacrimal gland of patients with chronic GVHD. Our findings strongly suggest that fibroblasts originated from circulating donor–derived precursors actively participate in the pathogenic process of lacrimal gland chronic GVHD.

Keywords: lacrimal gland • pathobiology • fluorescent in situ hybridization 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×