May 2005
Volume 46, Issue 13
ARVO Annual Meeting Abstract  |   May 2005
Analysis of Rabbit Lacrimal Gland Acinar Cells cultured on ECMP Coated Silicone Sheets
Author Affiliations & Notes
  • S. Selvam
    Chemical Engineering, University of Southern California, Los Angeles, CA
  • S.C. Yiu
    Ophthalmology, USC/Doheny Eye Institute, Los Angeles, CA
  • R.E. Smith
    Ophthalmology, USC/DEI, Los Angeles, CA
  • M.D. Trousdale
    DEI, Los Angeles, CA
  • C.A. Peng
    Chemical Engineering, University of Southern California, Los Angeles, CA
  • Z. Zhu
    DEI, Los Angeles, CA
  • D. Stevenson
    DEI, Los Angeles, CA
  • A.K. Mircheff
    Physiology and Biophysics, USC, Los Angeles, CA
  • T. Nakamura
    Physiology and Biophysics, USC, Los Angeles, CA
  • J.T. Jacob
    Ophthalmology, Louisiana State University Eye Center, New Orleans, LA
  • Footnotes
    Commercial Relationships  S. Selvam, None; S.C. Yiu, None; R.E. Smith, None; M.D. Trousdale, None; C.A. Peng, None; Z. Zhu, None; D. Stevenson, None; A.K. Mircheff, None; T. Nakamura, None; J.T. Jacob, None.
  • Footnotes
    Support  NEI Grants EY15457, EY03040 and grant from RPB
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4410. doi:
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      S. Selvam, S.C. Yiu, R.E. Smith, M.D. Trousdale, C.A. Peng, Z. Zhu, D. Stevenson, A.K. Mircheff, T. Nakamura, J.T. Jacob; Analysis of Rabbit Lacrimal Gland Acinar Cells cultured on ECMP Coated Silicone Sheets . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4410.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To study the response of rabbit lacrimal acinar cells to extracellular matrix protein (ECMP) coated and uncoated silicone sheets. Methods: Purified rabbit lacrimal gland acinar cells were obtained from aseptically excised inferior lacrimal glands using standard protocols. Biomedical grade silicone sheets were cut, sterilized and placed in wells of a 24–well tissue culture plate. The silicone in half of the plate wells (12 wells) was then coated with matrigel, an ECMP containing preparation; the remaining 12 wells served as controls with no coating. Cell suspensions (5 x 105/ml) were seeded into all wells and the plate cultured at 37ºC under 5% CO2 for 5 days. On culture day 5, three wells of each type were fixed with Karnovsky's fixative for at least 2 h at 4oC and washed with PBS for scanning electron microscopy (SEM) to evaluate the cellular morphologic properties. Additionally, on the same cell culture day the cellular physiologic properties were examined. Four wells of each material type, two wells with carbachol stimulation (stimulated) and two without carbachol (unstimulated), were used to evaluate secretion of catalytically active beta hexosaminidase. Results: SEM pictures showed cell clusters (acini) on both the matrigel–coated and control groups. The surfaces of the cell clusters had the characteristic appearance of the basal side of acini with no microvilli. Neither material had a cellular monolayer present at the end of cell culture. A significant increase in cell secretory activity was found in the cells with carbachol stimulation. But the degree of stimulation between the two material groups was not statistically different. Conclusions: Silicone is highly biocompatible to purified rabbit lacrimal gland acinar cells. It supports and maintains the lacrimal acinar phenotype and shows promise for use in a tissue engineered tear secretory system.

Keywords: lacrimal gland • extracellular matrix • cornea: tears/tear film/dry eye 

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