May 2005
Volume 46, Issue 13
ARVO Annual Meeting Abstract  |   May 2005
Lipase H cDNA in Human Lacrimal Gland
Author Affiliations & Notes
  • J.M. Crow
    Ophthalmology, Regions Hospital, St Paul, MN
  • J.D. Nelson
    Ophthalmology, Regions Hospital, St Paul, MN
  • S.G. Remington
    Ophthalmology, Regions Hospital, St Paul, MN
  • Footnotes
    Commercial Relationships  J.M. Crow, None; J.D. Nelson, None; S.G. Remington, None.
  • Footnotes
    Support  HealthPartners Research Foundation
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4419. doi:
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      J.M. Crow, J.D. Nelson, S.G. Remington; Lipase H cDNA in Human Lacrimal Gland . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4419.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: We asked whether mRNAs encoding members of the triacylglycerol lipase family were expressed in the human lacrimal gland. Methods: Poly(A)+ RNA was isolated from male human lacrimal gland tissue. (Human lacrimal gland tissue was made available by Dr. John Ubels.) We synthesized cDNA and amplified the cDNA with degenerate PCR primers based on two highly conserved sequence motifs in the triacylglycerol lipase family: RITGLD(P/A) and D(I/F)(Y/F)PNGG. We subcloned and sequenced the 180 base pair PCR products. Results: We compared the nucleotide sequences of human lacrimal gland PCR products with those in GenBank data bases. We identified PCR fragments representing lipoprotein lipase (LPL; accession # NM_000237) and lipase H (LIPH; accession# NM_139348), each members of the triacylglycerol lipase family. LipH is also known as phosphatidic acid–selective phospholipase A1 (PA–PLA1), an enzyme that converts phosphatidic acid to 2–acyl lysophosphatidic acid (LPA), a lipid mediator. Conclusions: Human lacrimal gland expressed cDNAs encoding LPL and LIPH. The LIPH enzyme, and/or its product LPA, may be secreted in the tears.

Keywords: gene/expression • lacrimal gland 

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