May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Th1/Th2 Cytokine Levels in the Tear Fluid of Patients With Sjogren’s Syndrome
Author Affiliations & Notes
  • H. Araki
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • E. Takamura
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • K. Sinozaki
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • M. Murata
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • K. Nishi
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • Y. Hirokawa
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • S. Hori
    Department of Ophthalmology, Tokyo Women's Medical University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  H. Araki, None; E. Takamura, None; K. Sinozaki, None; M. Murata, None; K. Nishi, None; Y. Hirokawa, None; S. Hori, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4464. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      H. Araki, E. Takamura, K. Sinozaki, M. Murata, K. Nishi, Y. Hirokawa, S. Hori; Th1/Th2 Cytokine Levels in the Tear Fluid of Patients With Sjogren’s Syndrome . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4464.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: The purpose of the study was to compare the expression of Th1 and Th2 cytokine levels in the tear fluid of normal eyes and those with Sj ö gren's syndrome (SS). Methods: Five patients with primary SS and 4 normal control subjects participated in this study. Tear fluid was obtained from the participants using Schirmer Tear Production Measuring StripsTM and soluble elucidates were used as tear fluid samples. The concentrations of IL–2, IL–4, IL–6, IL–10, TNF–α and IFN–γ were measured by BD Human Th1/Th2 Cytokine CBA KitTM in samples obtained from patients with primary SS and normal individuals. This kit combines the principles of the "sandwich" immunoassay with the capability of uniform–size microparticle–based flow cytometry for simultaneous measurement of six human cytokines in a single sample. Results: The mean concentration of IL–6 in the tears of patients with SS was elevated significantly compared to that of normal control subjects (179.0+/–124.3 vs. 25.5+/–10.7 pg/ml; Mann–Whitney U test: p=0.0010). Compared with normal subjects, high levels of IL–2, IL–4, IL–10, TNF–α and IFN–γ were detected in SS patients although not significant (p = 0.071, p =0.053, p =0.403, p = 0.076, and p =0.129, respectively) and only IL–6 levels reached statistical significance. Conclusions: Elevated tear fluid IL–6 levels in patients with SS suggest that IL–6 may contribute to the pathogenesis of SS, since it is known to facilitate the activation and differentiation of autoantibody secreting B cells.

Keywords: cornea: tears/tear film/dry eye • cytokines/chemokines • cornea: clinical science 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×