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M. Kivilcim, E. Aydin, G.A. Peyman, M. Riazi Esfahani, A.A. Kazi, D.R. Sanders; Combination of Triamcinolone Acetonide With Low Molecular Weight Heparin and Doxycycline in Inhibition of Experimental Corneal Angiogenesis . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4479.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the effect of combinations of topically administered triamcinolone acetonide and low molecular weight heparin (LMWH) or triamcinolone acetonide and doxycycline on experimental corneal neovascularization in rats in dosages that were ineffective when administered individually. Methods: Chemical cauterization of cornea in 24 eyes of 24 rats was performed by using silver nitrate/potassium nitrate sticks. The animals were anesthetized and treated in accordance with the ARVO statement on the use of animals in ophthalmic and visual research. Topical instillation of triamcinolone acetonide (20 µg/ml) and either low molecular weight heparin (LMWH, 10 mg/ml) or doxycycline (10 mg/ml) was compared to normal saline treatment for 7 days. Percentage area of cornea covered by neovascularization and scar in each group was calculated separately using computer software with digital photographs. Results: The means percent area of corneal neovascularization in triamcinolone acetonide and LMWH, triamcinolone acetonide and doxycycline, and control groups were 2.35 ± 4.42%, 9.42 ± 6.8%, 64.7 ± 10.0%, respectively. The means percent area of neovascularization in triamcinolone acetonide plus LMWH or triamcinolone acetonide plus doxycycline groups were significantly different from control group (p = 0.001, for both). There was no significant difference between study groups with regard to percent area of neovascularization, or percent area of corneal scar between control and study groups. Conclusions: Our study demonstrates that topically administered triamcinolone acetonide plus LMWH or triamcinolone acetonide plus doxycycline has effects which contribute to efficient suppression of corneal neovascularization when ineffective at similar concentrations alone. These angiostatic combinations can be therapeutically useful in the treatment of other ocular diseases.
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