May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Induction of Corneal Neovascularization by Matrix Metalloproteinases.
Author Affiliations & Notes
  • Q. Ebrahem
    Ophthalmic Research, Cole Eye Inst/Cleveland Clinic, Cleveland, OH
  • B. Anand–Apte
    Ophthalmic Research, Cole Eye Inst/Cleveland Clinic, Cleveland, OH
  • Footnotes
    Commercial Relationships  Q. Ebrahem, None; B. Anand–Apte, None.
  • Footnotes
    Support  NIH
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4482. doi:
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      Q. Ebrahem, B. Anand–Apte; Induction of Corneal Neovascularization by Matrix Metalloproteinases. . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4482.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Matrix metalloproteinases (MMPs) are a specialized group of enzymes that participate in extracellular matrix (ECM) degradation and have been postulated to play an important role in angiogenesis. The purpose of this study was to determine if the MMPs were involved upstream or downstream of VEGF in vivo. Methods: Hydron micropellets containing pro form or active form of MMPs (MMP–2 and MMP–9) were placed in micropockets in rat corneas and analyzed one week after implantation for their ability to induce a neovascular response. The ability of MMP inhibitors and VEGF neutralizing antibodies to inhibit the MMP as well as a VEGF mediated response was examined. Results: Active MMPs initiate an angiogenic response in the rat corneal assay in the form of growth of new vessels towards MMP loaded pellets. In addition, synthetic inhibitor of MMPs can inhibit the angiogenic response of VEGF. Conclusions: Active MMPs can initiate an angiogenic response that is mediated by release of low levels of VEGF. Our data suggests that MMPs may act upstream as well as downstream of VEGF during in vivo angiogenesis.

Keywords: retinal degenerations: cell biology • choroid • age-related macular degeneration 
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