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B. Bourghardt Peebo, L. Gan, C. Frennesson; Characteristics of New Vessels in a Rat Corneal Neovascularization Model . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4492.
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Purpose: To evaluate corneal neovascularization and subsequent spontaneous regression in a rat model. Methods: Corneal neovascularization was induced in 20 Dark Agouti rats, using intense diode laser (810 nm) photocoagulation of the fundus (90 laser burns, 200–220 mW, 125 µ m and 0.15 sec). The eyes were enucleated 7, 14, 28, 42 and 84 days after laser treatment. Paraffin sections were stained with eosin/hematoxylin and examined by light microscopy. To determine apoptosis, immunohistochemical studies with active caspase–3 antibodies, were performed. Proliferative activity was measured using proliferative cell nuclear antigen (PCNA) technique. Results: Neovascularization was seen from day 7 and reached a maximum on day 14. Regression with ghost vessels was observed at day 84. PCNA staining indicated maximal proliferative activity at day 14, after which a decrease in activity was seen throughout the 12 weeks. Using active caspase–3 antibodies, apoptosis was found from day 7 to day 84. Conclusions: In the present rat model, corneal neovascularization was demonstrated to start as a fairly rapid endothelial cell proliferation followed by a slow spontaneous regression through probable endothelial cell apoptosis and formation of ghost vessels.
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