May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
In situ Ablation of sFLT Disrupts the Corneal Anti–Angiogenic Barrier
Author Affiliations & Notes
  • B.K. Ambati
    Ophthalmology, Medical College of Georgia, Augusta, GA
  • N. Singh
    Ophthalmology, Medical College of Georgia, Augusta, GA
  • J. Witta
    Ophthalmology, University of Kentucky, Lexington, KY
  • M. Nozaki
    Ophthalmology, University of Kentucky, Lexington, KY
  • J.Z. Baffi
    Ophthalmology, University of Kentucky, Lexington, KY
  • B. Raisler
    Ophthalmology, University of Kentucky, Lexington, KY
  • S. Rashid
    Ophthalmology, Medical College of Georgia, Augusta, GA
  • T. Suthar
    Ophthalmology, Medical College of Georgia, Augusta, GA
  • A. Orecchia
    IDI, Rome, Italy
  • J. Ambati
    Ophthalmology, University of Kentucky, Lexington, KY
  • Footnotes
    Commercial Relationships  B.K. Ambati, None; N. Singh, None; J. Witta, None; M. Nozaki, None; J.Z. Baffi, None; B. Raisler, None; S. Rashid, None; T. Suthar, None; A. Orecchia, None; J. Ambati, None.
  • Footnotes
    Support  Knights Templar
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4501. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      B.K. Ambati, N. Singh, J. Witta, M. Nozaki, J.Z. Baffi, B. Raisler, S. Rashid, T. Suthar, A. Orecchia, J. Ambati; In situ Ablation of sFLT Disrupts the Corneal Anti–Angiogenic Barrier . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4501.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To determine if soluble FLT mediates corneal avascularity. Methods: A series of mouse and human corneal specimens were examined for the presence of soluble FLT. Binding of VEGF to sFLT was assessed by immunoprecipitation.In situ ablation of sFLT was performed in human cells and mouse corneas. Plasmid–directed replenishment of sFLT was performed to assess whether avascularity could be preserved. Results: sFLT is highly expressed in mouse and human corneal epithelium and also expressed in corneal stroma. It is downregulated in neovascularized human corneas. VEGF is sequestered by VEGF in normal cornea but much less so in neovascularized corneas. In situ ablation of sFLT breaches corneal avascularity in the mouse. These effects are effectively countered by plasmid–directed restoration of sFLT. Conclusions: sFLT mediates corneal avascularity.

Keywords: cornea: basic science • neovascularization • receptors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×