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T. Usui, S. Yamagami, S. Kishimoto, T. Mimura, K. Ono, S. Yokoo, S. Amano, S. Ishida, Y. Usui; Role of CD4+CD25+ Regulatory T Cells in Corneal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4503.
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Purpose:Because corneal neovascularization (NV) is closely associated with inflammation, leukocytes are assumed to play important roles in corneal NV. It has been recently suggested that CD4+CD25+ regulatory T cells have inhibitory effects in inflammatory pathological conditions. We have already reported that CD25+ T cells were involved in pathological retinal angiogenesis. Therefore, we investigate the roles of CD4+CD25+ regulatory T cells in corneal NV. Methods:Corneal NV was induced by suture (10–0 nylon) in corneal stroma of Balb/C mice (male, 8 weeks). Infiltrated CD4+CD25+ T cells in corneal NV areas were examined by immunohistochemistry of cross section and whole mount staining. To investigate the function of CD4+CD25+ T cells in corneal NV, monoclonal anti–CD25 neutralization antibodies (CD25 group, n = 8) and nonimmunized isotype control (control group, n = 8) were administered to mice, then corneal NV was induced by standard suturing technique with 10–0 nylon. NV areas were visualized by endothelial specific BS–1 lectin staining 7 days after surgery and measured. Results:CD4+CD25+ T cells were infiltrated in corneal NV, but not normal corneal area. Corneal NV was enhanced in the CD25 group as compared with that in the control group. Average corneal NV area in CD25 group was significantly larger than that in control group (p < 0.05). Conclusions:Our findings indicated that CD4+CD25+ T cells are involved in the corneal NV and may be a key factor to inhibit inflammatory corneal NV.
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