The Effects of EGF and TGFb to Corneal Endothelium Spreading

Abstract

Abstract: : Purpose: We examined the effects of epidermal growth factor(EGF) and transforming growth factor(TGFß) to corneal endothelium spreading. Methods: We used adult albino rabbit (n=17,body weigt2.5kg). An endothelial defect was produced by scraping in corneal brocks(4x4mm) obtained from adult albino rabbit. Corneal endothelium were examined in organ culture for 24 hours by adding EGF(10ng/ml), TGFß1(1ng/ml), TGFß2(1ng/ml), EGF(10ng/ml)+TGFß1(1ng/ml) or EGF(10ng/ml)+TGFß2(1ng/ml). Endothelial spreading was investigated by Arizalinin red. Results:EGF group promoted corneal endothelium spreading compared with control group (p<0.05). TGFß1, TGFß2, EGF+TGFß1 and EGF+TGFß2 group didn’t have significant difference compared with control group. EGF+TGFß1 and EGF+TGFß2 group inhibited corneal endothelium spreading compared with EGF group (p<0.05). Conclusions: EGF is required for promotion of corneal endothelium spreading. TGFß1 and TGFß2 is required for inhibition of the effect of EGF.

Keywords: cornea: endothelium • wound healing • growth factors/growth factor receptors