May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Chromosomal Abnormality of Cultivated Human Corneal Endothelial Cells and Keratocytes
Author Affiliations & Notes
  • T. Miyai
    Ophthalmology, Miyata Eye Hospital, Miyakonojo, Japan
  • Y. Maruyama
    Ophthalmology, Miyata Eye Hospital, Miyakonojo, Japan
  • Y. Osakabe
    Ophthalmology, Miyata Eye Hospital, Miyakonojo, Japan
  • R. Nejima
    Ophthalmology, Miyata Eye Hospital, Miyakonojo, Japan
  • K. Miyata
    Ophthalmology, Miyata Eye Hospital, Miyakonojo, Japan
  • S. Amano
    Department of Ophthalmology, University of Tokyo, Tokyo, Japan
  • Footnotes
    Commercial Relationships  T. Miyai, None; Y. Maruyama, None; Y. Osakabe, None; R. Nejima, None; K. Miyata, None; S. Amano, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4519. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T. Miyai, Y. Maruyama, Y. Osakabe, R. Nejima, K. Miyata, S. Amano; Chromosomal Abnormality of Cultivated Human Corneal Endothelial Cells and Keratocytes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4519.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To evaluate chromosome abnormalities of cultivated human corneal endothelial cells (HCEC) and cultivated human corneal keratocytes (HCK). Methods: Corneal endothelial cells with Descemet’s membrane or stroma removed from sclerocorneal pieces of human donor cornea were cultured on dishes coated with extracellular matrix of bovine corneal endothelial cells. Cell culture was performed to 5th passage. Chromosomal abnormalities of 20 specimens of HCEC (range 2–77 years old, average 43.2±25.0 years old) and 3 specimens of HCK (range 21–73 years old, average 54.7±29.2 years old) were examined with G–band karyotyping. Fifteen samples of HCEC were from passage 3 and 5 samples were from passage 5. Five samples of HCEC were continuously passaged without freezing and 15 samples were freezed during passaging. All samples of HCK were from passage 3 and they were passaged without freezing. Results: Mode number (mn) of chromosomes of HCEC distributed frm45 to 48. Four samples have mn of 45, 12 have mn of 46, 3 have mn of 47, and 1 has mn of 48. Main karyotypes of HCEC samples with mn45, 46, 47, and 48 were 45, X –X or –Y, 46, XX or Y, 47, XX,+8, 47,XX,+21, and 48, XX,+X,+20. Mode number of chromosomes of HCK was 45 for 1 specimen and 46 for 2 specimens. Main karyotypes of HCK samples with mn45 and 46 were 45, X –Y and 46 XX. Conclusions: Chromosomal abnormalities exist in cultivated HCEC and HCK. It should be elucidated whether these chromosomal abnormalities affect cellular function.

Keywords: cornea: endothelium • cornea: stroma and keratocytes • cornea: basic science 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×