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J.D. Ash, R.S. Charrad, R. Archer, M.E. Elliott; Caveolin 1 Is Essential to Maintain Blood Vessel Integrity in the Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4709.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Caveolin 1 (Cav–1) is an important membrane organizing protein that binds and regulates the function of essential angiogenic signaling molecules in vascular endothelial cells. Cav–1 is a negative regulation of VEGFR2, and eNOS, and is likely to play an important role in regulating vascular stability and angiogenesis. The purpose of this study was to determine the role of Cav–1 in regulating retinal vascular development and vascular stability in the oxygen induce retinopathy model. Methods: Retinas were dissected from mice reared in normal oxygen conditions, and from mice exposed to elevated oxygen. The retinal vasculature was stained using Griffionia simplicifilia lectin (isotype B4), and the retinas were flat mounted fro microscopy. For the oxygen exposure we placed Cav–1 knockout mice in 75% oxygen from postnatal day 7 to 13. Mice were then returned to room air until postnatal day 17. Neovascularization was quantified by counting preretinal vascular tufts on confocal images. Results: In room air control mice we observed that retinal vessels were significantly dilated compared to control mice. In the oxygen induced retinopathy model we observed similar levels of neovascularization in knockout and control mice. However, 100% of knockout mice had severe intra–retinal hemorrhaging while none of the control mice did. Conclusions: Cav–1 is an important regulator of vascular function and stability in the retina. The absence of Cav–1 results in a phenotype that is consistent with deregulated eNOS, and overstimulation of VEGFRs. The results suggest that Cav–1 may be an important target to regulate angiogenic signaling molecules.
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