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M. Papathanassiou, M. Lutolf, C. Futter, J. Hubbell, B. Allan; Immunoisolation in Corneal Endothelial Transplantation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4740.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To study the viability of endothelial cells after coating of pig corneas with a thin hydrogel barrier to immunoisolate the cornea. Methods: Corneoscleral buttons were prepared from fresh enucleated normal pig eyes. Biocompatible hydrogel barriers were formed by in situ polymerization of polyethylene glycol (PEG)–based precursors and used for coating of corneoscleral rims. Initial coating with detergent, pluronic, trying to achieve an homogenous thin hydrogel coating was also performed. Morphological integrity of the endothelial monolayer was studied by specular microscopy. Endothelial viability was assessed by live/dead staining and fluorescence microscopy. Results: Corneas can be coated successfully with thin clear hydrogel layers. Results of endothelial viability studies showed no significant difference between control corneas, corneas coated with hydrogel barriers only and corneas coated with diluted pluronic and hydrogels. Undiluted pluronic was toxic for endothelial cells. Conclusions: The development of techniques of posterior lamellar keratoplasty (DLEK) offers the possibility of complete tissue encapsulation of the transplanted endothelial layer. Immunoisolation coating is a promising tool for the prevention of graft rejections.
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