May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Immunoisolation in Corneal Endothelial Transplantation
M. Papathanassiou; M. Lutolf; C. Futter; J. Hubbell; B. Allan
Author Affiliations & Notes
  • M. Papathanassiou
    Cornea and External Diseases, Moorfields Eye Hospital, London, United Kingdom
  • M. Lutolf
    Microbiology and Immunology, Stanford University, Palo Alto, CA
  • C. Futter
    Department of Cell Biology, Institute of Ophthalmology, London, United Kingdom
  • J. Hubbell
    Institute for Biomedical Engineering, ETH Zurich, Zurich, Switzerland
  • B. Allan
    Cornea and External Diseases, Moorfields Eye Hospital, London, United Kingdom
  • Footnotes
    Commercial Relationships  M. Papathanassiou, None; M. Lutolf, None; C. Futter, None; J. Hubbell, None; B. Allan, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4740. doi:
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      M. Papathanassiou, M. Lutolf, C. Futter, J. Hubbell, B. Allan; Immunoisolation in Corneal Endothelial Transplantation . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4740.

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Abstract

Abstract: : Purpose: To study the viability of endothelial cells after coating of pig corneas with a thin hydrogel barrier to immunoisolate the cornea. Methods: Corneoscleral buttons were prepared from fresh enucleated normal pig eyes. Biocompatible hydrogel barriers were formed by in situ polymerization of polyethylene glycol (PEG)–based precursors and used for coating of corneoscleral rims. Initial coating with detergent, pluronic, trying to achieve an homogenous thin hydrogel coating was also performed. Morphological integrity of the endothelial monolayer was studied by specular microscopy. Endothelial viability was assessed by live/dead staining and fluorescence microscopy. Results: Corneas can be coated successfully with thin clear hydrogel layers. Results of endothelial viability studies showed no significant difference between control corneas, corneas coated with hydrogel barriers only and corneas coated with diluted pluronic and hydrogels. Undiluted pluronic was toxic for endothelial cells. Conclusions: The development of techniques of posterior lamellar keratoplasty (DLEK) offers the possibility of complete tissue encapsulation of the transplanted endothelial layer. Immunoisolation coating is a promising tool for the prevention of graft rejections.

Keywords: cornea: endothelium • intraocular pressure • transplantation 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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