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E. Baez, J.–Y. Hyon, C. Zhang, S. Hose, D. Sinha, T.P. O'Brien; In vivo Toxicity of Preserved and Self–Preserved Antibiotics in Rabbit Corneal Keratocytes . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4909.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the toxicity of preservative vs. self–preserved fourth–generation fluoroquinolones on corneal stromal keratocytes. Methods: Sixteen rabbits were randomly allocated to receive BSS®, VigamoxTM or ZymarTM. The corneal epithelium of the right eye was scraped from limbus to limbus using a surgical blade. The animals received two drops of medication before epithelial injury, 2 drops after epithelial injury and 1 drop every 15 minutes for 4 hours thereafter. The animals were sacrificed 4 hours after the initial epithelial injury. The eyes were then collected, fixed in 4% paraformaldehyde, transferred to a 20% sucrose buffer at 4ºC for cryoprotection and embedded. Two sections were counted and averaged per eye. TUNEL assays were performed on the tissue sections. Statistical analysis consisted of a Kruskal–Wallis test. Results: TUNEL positive cells (mean ± standard error) results were: BSS® = 64.8 ± 7.4; VigamoxTM = 81.3 ± 5.7 (P = 0.14); ZymarTM = 122.0 ± 8.7 (P = 0.08). The ZymarTM treatment group produced the highest number of TUNEL positive cells. Groups treated with topical antibiotics showed more TUNEL positive cells compared to the BSS® treated group but these differences were not statistically significant. Conclusions: There was a trend of increasing toxicity from BSS to self–preserved antibiotic and preserved antibiotic treated group, respectively, which did not achieve statistically significance. Topically administered fluoroquinolone antibiotics are generally safe, however, under frequent dosing conditions they may contribute to mild cytotoxicity in corneal cells.
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