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S. Bozbeyoglu, G. Take, D. Dursun, Y.A. Akova; Immunohistochemical Assessment of Topical Cyclosporine, Dexametasone and Diclofenac Effects in a Rat Corneal Wound Healing Model . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4911.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To evaluate the effects of topical treatment with cyclosporine–A (Cyc–A), dexametasone and diclofenac on the rat corneal wound healing model by using immunohistochemical examination. Methods: Thirty corneas of 15 Sprague–Dawley rats were used for the study. The corneas of the animals were divided among 5 groups. Group 1 was formed from 6 eyes of 3 rats, received neither operation nor treatment. Other 24 corneas of 12 rats received limbus to limbus epithelial scrapings. Group 2 was formed from 12 left corneas of these rats received no treatment. Group 3 was formed from 4 right corneas received Cyc–A %0.05, 4 times in a day for 7 days. Group 4 was formed from 4 right corneas received dexametasone % 0.1, 4 times in a day for 7 days. Group 5 was formed from 4 corneas received diclofenac sodium %0.1, 4 times in a day for 7 days. All rats were sacrified and their corneas were excised after 7 days. Immunological examinations were done for the cornea sections which were treated with 6 different antibodies as laminin–5 policlonal anti–rabbit Ig, fibronectin monoclonal anti–mouse IgG, integrin–ß1 policlonal anti–goad Ig, integrin–ß2 policlonal anti–goad Ig, MMP–2 polikconal anti–rabbit Ig ve MMP–9 monoklonal anti–rabbit Ig. These sections were evaluated by a histopathologist and the immunoreactive expressions of fibronectin, laminin–5, integrin–ß1, integrin–ß2, MMP–2 and MMP–9 were graded on a scale 0 to 3. Results were statistically compared for each drug group with its control group and the control groups with normals by using Wilcoxon Signed Ranks Test. Results:Dexametasone and Cyc–A created the same constructive effects by increasing the expressions of extracellular matrix proteins as fibronectin, laminin–5 and their receptors integrin–ß1, integrin–ß2. These effects were found statistically significant (p<0.05) for both drugs. Whereas their supressing effects on MMP–2 and MMP–9 expressions were found statistically insignificant (p>0.05). Diclofenac showed similar effects by stimulating the expression of fibronectin while supressing the expressions of MMP–2 and MMP–9. which was found statistically insignificant (p>0.05). However, 50 % of the rats treated with diclofenac, developed keratolysis. Conclusions: All drugs cumulative effects were thought to be affirmative on corneal wound healing by using immunohistochemical criteria. Keratoylsis developed in diclofenac group was explained as the possibility of different effects like other variants of MMP’s induction by this drug
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