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M.V. Netto, R.R. Mohan, S. Sinha, A. Sharma, S.E. Wilson; Prophylactic Mitomycin C Effect Corneal Haze and Cellularity . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4913.
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Purpose: Purpose: To compare the effectiveness and side effects of different exposure times and concentrations of topical mitomycin C (MMC) in preventing corneal haze after photorefractive keratectomy (PRK). Methods: PRK was performed to correct –9.0D of myopia in 32 New Zealand rabbits and animals were divided into four groups: PRK plus BSS (group I), PRK plus MMC 0.02% for 2 minutes (group II), PRK plus MMC 0.02% for 12 seconds (group III) and PRK plus MMC 0.002% for 1 minute (group IV). Rabbits were submitted to slit lamp analysis and haze grading and sacrificed at 4 weeks after surgery for subsequent staining for alpha–smooth–muscle actin (SMA) to detect myofibroblasts and DAPI to detect keratocytes. Results: Slit lamp grading showed a significant difference in haze formation between groups I and groups II, III, IV.(p < 0.05) There was also a statistically significant difference between group I (13.9 cells/400X field ± 5.6 SD) compared with groups II (1.2 ± 0.6), III (2.1 ± 2.2) and IV (2.6 ± 2.4) for cells staining with SMA (p < 0.01). There was no statistically significant difference between groups II, III and IV for cells staining with SMA. A significant reduction in keratocyte density in the anterior stroma was noted in groups II (92.4 ± 24), groups III (108.6 ± 26), and IV (122.4 ± 30) treated with mitomycin C compared to the control group I (141.8 ± 28) at four weeks after surgery. The differences between groups II, III, and IV were statistically significant.(p < 0.05) Conclusions: Prophylactic Mitomycin C significantly decreased corneal haze and myofibroblast generation at one month after PRK for high myopia in a rabbit model. Decreased haze and myofibroblast density were noted at both high and low concentrations and with lower exposure times at the higher concentration. Decreased keratocyte density in the anterior stroma was noted in all groups treated with MMC, especially following the higher concentrations (0.02%) with longer exposure, but the decrease in keratocyte density was less with lower concentration treatment. EY10056
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