May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Mitomycin C Changes the Inflammatory Cytokines Expression of Porcine Corneal Fibroblasts
S.–W. Chang; S.–F. Chou; Y.–H. Wang
Author Affiliations & Notes
  • S.–W. Chang
    Dept Ophthal, Far Eastern Mem Hosp, Ban–Chiao, Taiwan Republic of China
    Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan Republic of China
  • S.–F. Chou
    Dept Ophthal, Far Eastern Mem Hosp, Ban–Chiao, Taiwan Republic of China
  • Y.–H. Wang
    Dept Ophthal, Far Eastern Mem Hosp, Ban–Chiao, Taiwan Republic of China
  • Footnotes
    Commercial Relationships  S. Chang, None; S. Chou, None; Y. Wang, None.
  • Footnotes
    Support  NSC 93–2314–B–418–002
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4914. doi:
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      S.–W. Chang, S.–F. Chou, Y.–H. Wang; Mitomycin C Changes the Inflammatory Cytokines Expression of Porcine Corneal Fibroblasts . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4914.

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Abstract

Abstract: : Purpose: To investigate the expression of inflammatory cytokines mRNA expression following mitomycin C (MMC) application in porcine corneal fibroblasts. Methods: Primary porcine corneal fibroblasts, passages 3–5, were treated with MMC at concentrations of 0.2mg/ml for 1, 2, and 5 minutes. Control corneal fibroblasts were treated with DMEM. Morphological changes were documented with phase contrast microscopy and compared at 0, 24, 48, 72 hours following treatment. The effects of mitomycin C on cell morphology and cell growth were analyzed by phase–contrast microscopy and PicoGreeen Assay respectively. Cell viability was studied by staining with annexin V–FITC/PI and analyzed flow cytometry. The relative expression of mRNA of interleukin–1 (IL–1), IL–8, monocyte chemoattractant protein–1 (MCP–1), and transforming growth factor–beta 1 (TGF–b1) were investigated with real–time polymerase chain reaction. Results: MMC did not cause significant corneal fibroblast death but retarded cell growth as compared to control group. The expression of IL–1, IL–8, and MCP–1 were upregulated in a treatment time dependent pattern, while the expression of TGF–b1 mRNA did not change significantly. Conclusions: MMC changed the corneal fibroblast morphology and upregulated IL–1, IL–8, and MCP–1 mRNA expression.

Keywords: wound healing • cornea: stroma and keratocytes • cytokines/chemokines 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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