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S.–W. Chang, S.–F. Chou, Y.–H. Wang; Mitomycin C Changes the Inflammatory Cytokines Expression of Porcine Corneal Fibroblasts . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4914.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the expression of inflammatory cytokines mRNA expression following mitomycin C (MMC) application in porcine corneal fibroblasts. Methods: Primary porcine corneal fibroblasts, passages 3–5, were treated with MMC at concentrations of 0.2mg/ml for 1, 2, and 5 minutes. Control corneal fibroblasts were treated with DMEM. Morphological changes were documented with phase contrast microscopy and compared at 0, 24, 48, 72 hours following treatment. The effects of mitomycin C on cell morphology and cell growth were analyzed by phase–contrast microscopy and PicoGreeen Assay respectively. Cell viability was studied by staining with annexin V–FITC/PI and analyzed flow cytometry. The relative expression of mRNA of interleukin–1 (IL–1), IL–8, monocyte chemoattractant protein–1 (MCP–1), and transforming growth factor–beta 1 (TGF–b1) were investigated with real–time polymerase chain reaction. Results: MMC did not cause significant corneal fibroblast death but retarded cell growth as compared to control group. The expression of IL–1, IL–8, and MCP–1 were upregulated in a treatment time dependent pattern, while the expression of TGF–b1 mRNA did not change significantly. Conclusions: MMC changed the corneal fibroblast morphology and upregulated IL–1, IL–8, and MCP–1 mRNA expression.
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