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T. Rodriguez–Ares, M.J. López– Valladares, R. Tourino Peralba, B. Vieites, M. Silva, J. Couceiro; Ultra Structure of Lyophilized Human Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4977.
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Purpose: To describe the effect of lyophilization in amniotic membrane ultra structure, to provide esperimental evidence for clinical use. Methods: Human placentas were obtained after elective cesarean deliveries from donors seronegative. After being washed and separated from chorion, amniotic membrane was laid onto a cellulose nitrate filter paper and cut into pieces. These pieces went under lyophilization (freeze–dryer VIRTIS Genesis). Small samples of lyophilized human amniotic membrane were fixed in 2 % glutaraldehyde and examined with a transmission electron microscope. Results: Examination of the lyophilized human amniotic membrane by electron microscope confirmed that the tissue was well preserved and the basement membrane was clearly visible in all cases. Conclusions: Lyophilized human amniotic membrane maintains the ultra structural characteristics of this tissue including the presence of a clear basement membrane. Consequently lyophilization is a good alternative to cryopreservation for amniotic membrane, though more studies are necessary to confirm the usefulness of lyophilized human amniotic membrane in ocular surface reconstruction. Grant Identification: FIS – PI021608
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