May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Amniotic Membrane– Poly (Vinyl Alcohol) Hybrid Polymer as an Artificial Cornea Scaffold
Author Affiliations & Notes
  • Y. Uchino
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • H. Miyashita
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • S. Shimmura
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • H. Kobayashi
    Biomaterials Center, National Institute for Materials Science, Tukuba, Japan
  • J. Shimazaki
    Ophthalmology, Tokyo Dental College, Ichikawa, Japan
  • K. Tsubota
    Ophthalmology, Keio University, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Y. Uchino, None; H. Miyashita, None; S. Shimmura, None; H. Kobayashi, None; J. Shimazaki, None; K. Tsubota, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4986. doi:
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      Y. Uchino, H. Miyashita, S. Shimmura, H. Kobayashi, J. Shimazaki, K. Tsubota; Amniotic Membrane– Poly (Vinyl Alcohol) Hybrid Polymer as an Artificial Cornea Scaffold . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4986.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the biocompatibility of an amniotic membrane–immobilized polyvinyl alcohol (AM–i–PVA) hydrogel for use as an artificial cornea material. Methods: Rabbit limbal epithelial cells were air–lift cultured on AM–i–PVA discs with mitomycin–treated 3T3 feeder cells for 1 week. Differentiation of the epithelium and basement membrane formation were examined by immunohistochemistry for K3 (AE5), occludin, collagen IV and collagen VII. In an animal study, a 6 mm diameter AM–i–PVA disc was implanted into stromal pockets of Japanese white rabbits (n=12). An epithelial window (3 mm) was created over the implanted AM–i–PVA to allow epithelialization. The rabbits were followed for 1 month by slit lamp photography, followed by histological examination with HE stains. Results: Rabbit limbal cells formed 3 to 5 layers of keratin–3–positive epithelium on AM–i–PVA. Occludin (+) cells were observed lining the superficial epithelium, while both collagen IV and VII were observed in the basement membrane. Epithelization over implanted AM–i–PVA was complete within 2 weeks, with little inflammation or opacification of the hydrogel. Conclusions: AM–i–PVA supports a fully stratified, differentiated epithelium in vitro, and allows epithelial migration when transplanted in vivo.

Keywords: cornea: basic science • cornea: stroma and keratocytes • immunohistochemistry 
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