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M.C. Wathier, M.A. Carnahan, P.J. Jung, T. Kim, M.W. Grinstaff; Dendrimer Hydrogels as New Alternative for the Repair of Clear Corneal Incisions . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4993.
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Purpose:We investigated the ability of a new dendritic hydrogel adhesive to seal clear corneal incisions–the wound created during cataract procedures. Methods: To determine if this hydrogel adhesive can secure a clear corneal incisions, we performed several in vitro experiments on enucleated eyes. A 3.0 mm dual beveled, angled slit knife was used to make a 3.0 mm clear corneal linear incision. This wound was then left to self–seal or closed using either one interrupted 10–0 nylon suture or the hydrogel sealant. 4 µL of the hydrogel was applied to the incision and the polymer gelled within 4 seconds closing the wound. After 10 minutes, saline was then injected in the anterior chamber (8 mL/hr) until the repaired incision leaked. A cardiac transducer probe monitored the leaking pressure for the untreated or self–seal (N=7), nylon suture (N = 3) and hydrogel sealant (N = 8) treated eyes. Results: In this in vitro study, the mean leaking pressure (LP) for the self–seal and suture treated eyes were 24 ± 8 and 54 ± 16 mm Hg, respectively while the LP was 184 ± 79 mm Hg for the hydrogel sealant. Conclusions: A new in–situ polymerizing hydrogel is reported that secures 3 mm clear corneal incisions. The hydrogel sealant forms within less than a minute. The use of a sealant as opposed to nylon sutures or the self–seal approach in cataract surgeries provides a facile method to safely secure the wound and reduce potential complications. These results further support the synthesis and evaluation of dendritic macromolecules as hydrogel adhesives for ophthalmologic applications. Support This work was supported by the Johnson and Johnson Focused Giving Program and the NIH. We thank the North Carolina Eye Bank.
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