May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
A Modified Chondroitin Sulfate Aldehyde Adhesive for Sealing Corneal Incisions
J.G. Reyes; S. Herretes; A. Pirouzmanesh; D.–A. Wang; J. Elisseeff; A. Jun; P.J. McDonnell; R.S. Chuck; A. Behrens
Author Affiliations & Notes
  • J.G. Reyes
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • S. Herretes
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • A. Pirouzmanesh
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • D.–A. Wang
    Biomedical Engineering, Johns Hopkins University, Baltimore, MD
  • J. Elisseeff
    Biomedical Engineering, Johns Hopkins University, Baltimore, MD
  • A. Jun
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • P.J. McDonnell
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • R.S. Chuck
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • A. Behrens
    Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD
  • Footnotes
    Commercial Relationships  J.G. Reyes, None; S. Herretes, None; A. Pirouzmanesh, None; D. Wang, None; J. Elisseeff, None; A. Jun, None; P.J. McDonnell, None; R.S. Chuck, None; A. Behrens, None.
  • Footnotes
    Support  NIH Grant R21EB002369 (JHE)
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 4998. doi:
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      J.G. Reyes, S. Herretes, A. Pirouzmanesh, D.–A. Wang, J. Elisseeff, A. Jun, P.J. McDonnell, R.S. Chuck, A. Behrens; A Modified Chondroitin Sulfate Aldehyde Adhesive for Sealing Corneal Incisions . Invest. Ophthalmol. Vis. Sci. 2005;46(13):4998.

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Abstract

Abstract: : Purpose: To compare a modified chondroitin sulfate–aldehyde adhesive with standard sutures for sealing corneal incisions. Methods: A keratome knife was used to create non self–sealing uniplanar 3 mm clear corneal incisions in enucleated rabbit eyes (n=18). The wounds were sealed with either a chondroitin sulfate–aldehyde adhesive (n=8), three 10–0 nylon sutures (n=5) or one 10–0 nylon suture (n=5). Wound stability was tested by filling the globes with balanced salt solution through an anterior chamber port and slowly increasing the intraocular pressure. The pressure changes were monitored using a digital manometer connected to the anterior chamber and leak pressure was recorded for each eye. Confocal microscopy was performed on the glued eyes to document the glue distribution along the wound. Results: The mean leak pressures in the single–suture and three–suture subgroups were 26.4 mmHg (SD 6.0) and 44.3 mmHg (SD 8.2), respectively. The maximum intraocular pressure achieved in eyes that received the glue was 104.7 mmHg with a mean of 101.4 mmHg (SD 3.2). None of the eyes using glue showed leakage. At confocal microscopy, the glue was distributed inside the wound edges as a homogeneous thin layer of a less dense signal than stroma. Conclusions: A novel chondroitin sulfate–aldehyde adhesive was proven to be effective ex vivo for sealing corneal incisions in rabbit eyes and was superior to sutures for this purpose.

Keywords: cornea: basic science • clinical laboratory testing 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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