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X.–L. Han; Role of Tissue–Type Plasminogen Activator and Its Inhibitor in the Early Corneal Alkaline Burn With Amniotic Membrane Transplantation in Rabbit . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5045.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the role of tissue–type plasminogen activator (t–PA) and plasminogen activator inhibitor I (PAI–I) in the early corneal alkali burn with amniotic membrane transplantation (AMT) in rabbit with corneal alkali burn. Methods: 54 of New Zealand white rabbits were used and corneal alkali burn were made. Rabbits were divided into three groups: corneal alkaline burn only as control (C) group, fresh amniotic membrane transplantation group (fAMT), and preserved amniotic membrane transplantation group (pAMT). AMT was performed 30 minutes after corneal alkali burn. Pathohistology and computerized corneal analysis were studied at day 14, 30, and 60. Results: Compared with C, t–PA activity was significantly (P<0.01) reduced in fAMT and pAMT at all time. Total PAI–I activity was significantly (P<0.01) reduced at day 14 in fAMT and pAMT, respectively, and only significantly decreased at day 30 in fAMT. t–PA/PAI–I was significantly (P<0.05) reduced in AMT. At day 14, t–PA activity was significantly (P<0.05) lower in fAMT, but total PAI–I activity was higher than that in pAMT. t–PA/PAI–I was lower in fAMT than that of pAMT. Conclusions: Our results demonstrate that decrease in t–PA activity and t–PA/PAI may play an important role in the healing process of early corneal alkali burn treated with AMT.
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