May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Toll–Like Receptor (TLR) Expression in Oral Ulcer Biopsies From Behçet's Disease (BD) Patients: A Role for the Innate Immune System
Author Affiliations & Notes
  • P.I. Murray
    Academic Unit of Ophthalmology, Birmingham & Midland Eye Ctr, The University of Birmingham, Birmingham, United Kingdom
  • O.M. Durrani
    Academic Unit of Ophthalmology, Birmingham & Midland Eye Ctr, The University of Birmingham, Birmingham, United Kingdom
  • J. Hamburger
    The School of Dentistry, The University of Birmingham, Birmingham, United Kingdom
  • H. Williams
    Department of Pathology, The University of Birmingham, Birmingham, United Kingdom
  • F. Fortune
    Department of Oral Medicine, Queen Mary University of London, United Kingdom
  • J. Stewart
    Department of Oral Medicine, Queen Mary University of London, United Kingdom
  • G.R. Wallace
    Academic Unit of Ophthalmology, Birmingham & Midland Eye Ctr, The University of Birmingham, Birmingham, United Kingdom
  • Footnotes
    Commercial Relationships  P.I. Murray, None; O.M. Durrani, None; J. Hamburger, None; H. Williams, None; F. Fortune, None; J. Stewart, None; G.R. Wallace, None.
  • Footnotes
    Support  None.
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 5093. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P.I. Murray, O.M. Durrani, J. Hamburger, H. Williams, F. Fortune, J. Stewart, G.R. Wallace; Toll–Like Receptor (TLR) Expression in Oral Ulcer Biopsies From Behçet's Disease (BD) Patients: A Role for the Innate Immune System . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5093.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose:It has been proposed that infection at mucosal sites leads to chronic activation of the immune response, which in turn induces the widespread vasculitis and tissue damage seen in BD. However, the link between mucosal ulceration and activation of the immune response is not yet elucidated. TLRs are key molecules involved in the recognition of invading pathogens by the innate immune system. We wished to investigate the expression of TLRs in oral biopsies from patients with BD. Methods:Immunohistochemical analysis was used to examine the expression of TLRs 2, 4, 6, 9 & 10 in mouth ulcer biopsies from BD patients as compared with healthy oral mucosa, and aphthous ulcer and lichen planus biopsies acting as disease controls. To determine the nature of cells expressing the TLRs, duel staining with CD4, CD8, CD56 and CD68 was performed. Results:TLRs were present in all tissues examined and primarily expressed by macrophages, polymorphonuclear and mast cells. Significant differences in the intensity and the cells expressing the TLRs were noted between the BD oral biopsies and the control tissues, with increased expression in BD samples with positive staining in the epithelial layers (not seen in controls). The intensity of expression of TLRs in descending order was TLR 4>2>10>9>6. Initial studies suggest that CD68 macrophages are the prime source of TLR in these tissues. Conclusions:There appears to be an increased expression of TLRs in BD oral ulcer biopsies. This may result in an exaggerated immune response to invading pathogen, but over–expression of TLRs due to downstream signalling failure cannot be excluded.

Keywords: inflammation • immunohistochemistry • microbial pathogenesis: experimental studies 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×