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P.I. Murray, O.M. Durrani, J. Hamburger, H. Williams, F. Fortune, J. Stewart, G.R. Wallace; Toll–Like Receptor (TLR) Expression in Oral Ulcer Biopsies From Behçet's Disease (BD) Patients: A Role for the Innate Immune System . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5093.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:It has been proposed that infection at mucosal sites leads to chronic activation of the immune response, which in turn induces the widespread vasculitis and tissue damage seen in BD. However, the link between mucosal ulceration and activation of the immune response is not yet elucidated. TLRs are key molecules involved in the recognition of invading pathogens by the innate immune system. We wished to investigate the expression of TLRs in oral biopsies from patients with BD. Methods:Immunohistochemical analysis was used to examine the expression of TLRs 2, 4, 6, 9 & 10 in mouth ulcer biopsies from BD patients as compared with healthy oral mucosa, and aphthous ulcer and lichen planus biopsies acting as disease controls. To determine the nature of cells expressing the TLRs, duel staining with CD4, CD8, CD56 and CD68 was performed. Results:TLRs were present in all tissues examined and primarily expressed by macrophages, polymorphonuclear and mast cells. Significant differences in the intensity and the cells expressing the TLRs were noted between the BD oral biopsies and the control tissues, with increased expression in BD samples with positive staining in the epithelial layers (not seen in controls). The intensity of expression of TLRs in descending order was TLR 4>2>10>9>6. Initial studies suggest that CD68 macrophages are the prime source of TLR in these tissues. Conclusions:There appears to be an increased expression of TLRs in BD oral ulcer biopsies. This may result in an exaggerated immune response to invading pathogen, but over–expression of TLRs due to downstream signalling failure cannot be excluded.
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