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C. Kanno, Y. Kashiwagi, K. Horie, M. Inomata, T. Imamura, T. Yamamoto, H. Yamashita; Signal Transduction Pathway of ActivinA in Retinoblastoma Cell Lines . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5171.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: ActivinA is a member of the transforming growth factor (TGF) – beta superfamily and exerts certain effects on neurons. In this study, the signal transduction pathway of activinA was investigated in retinoblastoma (RB) cell lines. Methods: RB cell lines, Y79 and WERI–Rb–1 were used as the undifferentiated retinal cells. The expression of activin receptors (type IA, type IB, type II and type IIB) and cytoplasmic components for signal transduction, Smads (Smad2, Smad3, Smad4, Smad6 and Smad7) was investigated by RT–PCR. The morphological changes of retinoblastoma cells were observed after the stimulation by activinA. The signal transduction pathway was analyzed by using Luciferase assay with ARE–lux construct and by detecting the translocation of Smads after the stimulation of activinA. Results: All the activin receptors, Smad2, Smad3, Smad4, Smad6 and Smad7 were detected in both cell lines. Conclusions: The activinA signal was shown to be transmitted through Smads in the retinoblastoma cell lines.
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