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A. Mankowska, R.A. Rejdak, T. Zarnowski, E. Zielinska, K. Nowomiejska, K. Rejdak, P. Grieb, Z. Zagorski; Vitreous Nitrite/Nitrate Concentrations Are Increased in Patients With Retinal Detachement and Malignant Glaucoma . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5182.
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Purpose: The assessment of NO stable end products, nitrite and nitrate (NOx), is a reliable index of endogenous NO production in biological fluids (Rejdak K et al., Neurology, 2004). NOx levels were reported to be increased in diabetic retinopathy but little is known on the involvement of NO production in the pathogenesis of other retinal diseases. This study was undertaken to investigate this issue in aspect of retinal detachement (RD), malignant glaucoma (MG) and macular holes (MH). Methods: Vitreous fluid samples were obtained at the time of vitreoretinal surgery from 20 patients with RD, and 5 patients with MG and 8 with MH. Vitreous fluid samples from healthy eyes of organ donors obtained within 4 hours most mortem were used as a control. Total nitrite/nitrate (NOx) concentration was measured by a vanadium–based assay with the colorimetric Griess reaction (Rejdak K et al., J Neurol Sci 2003). Results: NOx levels were 13.0 ± 1.7 microM and 43.5 ± 6 microM in the vitreous of patients with RD and MG, respectively. In 6 cadaver eyes NOx content was of 1.6 ± 0.3 and eight vitreous samples from patients who underwent macular hole surgery, mean NOx level was 2.3 ± 0.2. The intravitreous concentration of NOx was significantly elevated in patients with RD (p.=0.0014), MG (p.<0.0001) but not in MH (p.=0.06) as compared to the control group. Conclusions: Vitreal NOx concentrations are increased in patients with retinal detachment and malignant glaucoma. Our results suggest that enhanced endogenous NO synthesis might be involved in the pathomechanisms of hypoxic/ischemic insult of inner and outer retina and should be considered as a potential pathway of pharmacological intervention.
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