May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
siRNA Connective Tissue Growth Factor (CTGF) Transfection of RPE Cells Decrease the Gene Expression of CTGF
S. Hoffmann; N. Zhang; S. He; S. Ryan; D. Hinton
Author Affiliations & Notes
  • S. Hoffmann
    Ophthalmology,
    Doheny Eye Institute, University of Southern California, Los Angeles, CA
  • N. Zhang
    Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA
  • S. He
    Ophthalmology,
    Doheny Eye Institute, University of Southern California, Los Angeles, CA
  • S. Ryan
    Ophthalmology,
    Doheny Eye Institute, University of Southern California, Los Angeles, CA
  • D. Hinton
    Ophthalmology and Pathology,
    Doheny Eye Institute, University of Southern California, Los Angeles, CA
  • Footnotes
    Commercial Relationships  S. Hoffmann, None; N. Zhang, None; S. He, None; S. Ryan, None; D. Hinton, None.
  • Footnotes
    Support  NIH grants EY03040 and EY01545, Research to Prevent Blindness and the Arnold and Mabel Beckman Found
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 5204. doi:
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      S. Hoffmann, N. Zhang, S. He, S. Ryan, D. Hinton; siRNA Connective Tissue Growth Factor (CTGF) Transfection of RPE Cells Decrease the Gene Expression of CTGF . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5204.

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Abstract

Abstract: : Purpose:To investigate the feasibility of CTGF gene modulation by use of small interfering RNA (siRNA) on retinal pigment epithelial cells (RPE). Methods: Subconfluent human RPE cells were transfected with si RNA CTGF in a concentration range of 50 nM, 150 nM and 300 nM for 48 hours. After this time course, the cells were lysed and a Western Blot assay was performed with a polyclonal CTGF antibody. Results: CTGF gene expression was reduced by si RNA CTGF in a dose dependent fashion. siRNA CTGF reduced CTGF gene expression by 60 %. A higher concentration of 300 nM diminished the gene expression of CTGF by 70 %.Conclusions:CTGF is a novel growth factor involved in the promotion of angiogenesis and wound healing. Therefore, CTGF siRNA may be feasible to inhibit proliferative and also neovascular diseases of the retina promoted by RPE cells.

Keywords: growth factors/growth factor receptors • gene modifiers • retinal pigment epithelium 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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