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S. Hoffmann, N. Zhang, S. He, S. Ryan, D. Hinton; siRNA Connective Tissue Growth Factor (CTGF) Transfection of RPE Cells Decrease the Gene Expression of CTGF . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5204.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To investigate the feasibility of CTGF gene modulation by use of small interfering RNA (siRNA) on retinal pigment epithelial cells (RPE). Methods: Subconfluent human RPE cells were transfected with si RNA CTGF in a concentration range of 50 nM, 150 nM and 300 nM for 48 hours. After this time course, the cells were lysed and a Western Blot assay was performed with a polyclonal CTGF antibody. Results: CTGF gene expression was reduced by si RNA CTGF in a dose dependent fashion. siRNA CTGF reduced CTGF gene expression by 60 %. A higher concentration of 300 nM diminished the gene expression of CTGF by 70 %.Conclusions:CTGF is a novel growth factor involved in the promotion of angiogenesis and wound healing. Therefore, CTGF siRNA may be feasible to inhibit proliferative and also neovascular diseases of the retina promoted by RPE cells.
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