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S.–J. Park, E.–J. Lim, H.–J. Kim, K.–R. Choi, S.–J. Oh, M.–H. Chun; The Dystrophic Clathrin–Dependent Endocytosis of Rod Bipolar Cells in the FVB/N Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5257.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the changes of clathrin–dependent endocytosis at the axon terminal of rod bipolar cells in rd1 type model of retinitis pigmentosa, FVB/N mouse. Methods: The retinas of FVB/N mice at postnatal day (P) 12, 14, 16, 21 and 49 were immunostained with monoclonal antisera against clathrin (1:200,000, Chemicon) and dynamin (1:20,000, Chemicon) which are the markers of synaptic vesicle endocytosis. For double labeling, polyclonal antisera against PKCα (1:1,000, Santa Cruz), rod bipolar cell marker, was used. Results: From P12, photoreceptor cells started to degenerate, and at P21 the outer nuclear layer (ONL) consisted of 2–3 cell layers. In the control group, each clathrin and dynamin immunoreactivity expressed from P14 to P49 at the axon terminal of PKCα labeled rod bipolar cells. In the FVB/N group, each clathrin and dynamin immunoreactivity was not visible in all terminals of rod bipolar cells at P14 and disappeared by P21. Conclusions: In the FVB/N mouse retina, rod bipolar cells are devoid of clathrin–dependent endocytosis .
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