May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
The Dystrophic Clathrin–Dependent Endocytosis of Rod Bipolar Cells in the FVB/N Mouse Retina
Author Affiliations & Notes
  • S.–J. Park
    Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • E.–J. Lim
    Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • H.–J. Kim
    Ophthalmology, College of Medicine, Ewa woman's University, Seoul, Republic of Korea
  • K.–R. Choi
    Ophthalmology, College of Medicine, Ewa woman's University, Seoul, Republic of Korea
  • S.–J. Oh
    Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • M.–H. Chun
    Anatomy, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  S. Park, None; E. Lim, None; H. Kim, None; K. Choi, None; S. Oh, None; M. Chun, None.
  • Footnotes
    Support  Brain Korea 21Grant
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 5257. doi:
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      S.–J. Park, E.–J. Lim, H.–J. Kim, K.–R. Choi, S.–J. Oh, M.–H. Chun; The Dystrophic Clathrin–Dependent Endocytosis of Rod Bipolar Cells in the FVB/N Mouse Retina . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5257.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the changes of clathrin–dependent endocytosis at the axon terminal of rod bipolar cells in rd1 type model of retinitis pigmentosa, FVB/N mouse. Methods: The retinas of FVB/N mice at postnatal day (P) 12, 14, 16, 21 and 49 were immunostained with monoclonal antisera against clathrin (1:200,000, Chemicon) and dynamin (1:20,000, Chemicon) which are the markers of synaptic vesicle endocytosis. For double labeling, polyclonal antisera against PKCα (1:1,000, Santa Cruz), rod bipolar cell marker, was used. Results: From P12, photoreceptor cells started to degenerate, and at P21 the outer nuclear layer (ONL) consisted of 2–3 cell layers. In the control group, each clathrin and dynamin immunoreactivity expressed from P14 to P49 at the axon terminal of PKCα labeled rod bipolar cells. In the FVB/N group, each clathrin and dynamin immunoreactivity was not visible in all terminals of rod bipolar cells at P14 and disappeared by P21. Conclusions: In the FVB/N mouse retina, rod bipolar cells are devoid of clathrin–dependent endocytosis .

Keywords: retina • retinal degenerations: cell biology • bipolar cells 
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