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P.K. Russ, C. Pino, A. Jayagopal, R. Haselton; Investigation of a New Potential Mouse Model of Age–Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5294.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate a new potential model of age– related macular degeneration (AMD) for use in future research studies. Methods: Transgenic mice expressing exogenous klotho protein and wild type animals were obtained from Makoto Kuro–o (UT Southwestern). Fluorescein microangiography was performed on animals 67–83 weeks in age. Mice were then euthenized, and their eye were embedded, sectioned and H & E stained. Stained sections were examined for pathology similar to that seen in patients with age–related macular degeneration. Results: Approximately 30% of klotho mice examined exhibited significant retinal vascular pathology, including tortuous veins and capillary fall–out seen in branch retinal vein occlusion. Paraffin embedded sections of the eyes from these mice also revealed significant degeneration of the neural retina. In addition, infiltration of the retina by the pigmented epithelium, a characteristic of macular degeneration, was also seen. The remaining klotho mice and two control mice exhibited possible microaneurysms and increased venous tortuosity that could be characterized as mild background retinopathy. The remaining wild type mice appeared to have relatively normal retinal vasculatures and normal neural retinal architecture. Conclusions: We have found a potential new mouse model of age–related macular degeneration. These results indicate the potential of further studies to validate the model which could then be used in future studies of the mechanisms involved in the development of AMD.
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