Purchase this article with an account.
E.–J. Lim, S.–J. Park, S.–J. Oh, M.–H. Chun; Na–K–Cl Cotransporter 2 Immunoreactive Cells in the Guinea Pig Retina . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5352.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: Na–K–Cl cotransporter 2 (NKCC2) is a class of ion transport proteins that transport Na, K, Cl ions into and out of cells in an electrically neuronal manner. This study was conducted to characterize NKCC2–labeled cells in the guinea pig retina using immunocytochemistry. Methods: Five retinas of adult guinea pig were used. Retinas were immunostained with polyclonal antisera against NKCC2 (1:1000, Chemicon) using indirect immunoflorescence. Results: NKCC2 immunoreactivity was present in horizontal cell bodies and their processes in the outer plexiform layer. Certain populations of bipolar cells were also labeled, and the axon terminals of these bipolar cells terminate in OFF sublamina of the inner plexiform layer. In addition, ganglion cells and displaced amacrine cells in the ganglion cell layer were also labeled. Conclusions: NKCC2 may transport Na, K, Cl ions in certain retinal neurons in the guinea pig retina.
This PDF is available to Subscribers Only