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H. Bando, Y. Ikuno, K. Sayanagi, Y. Tano; Platelet–Derived Growth Factor Up–Regulates Integrin 1 and 2 Expression Mediated by Mitogen Activated Protein Kinase (MAPK) in ARPE–19 Cell . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5361.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: : Cell–mediated gel contraction force is a contributing factor to proliferative vitreoretinopathy (PVR). We presented the reverse transcriptase polymerase chain reaction (RT–PCR) results, indicating that platelet–derived growth factor (PDGF) up–regulated mRNA expression of integrin α2 via mitogen–activated protein kinase (MAPK) at 2002 ARVO meeting. We currently investigate whether PDGF affects on expression level of integrin subtypes and if MAPK or phosphatidylinositol 3–kinase (PI3K) is involved in cultured ARPE–19. Methods: Gel contraction assay was performed in cultured ARPE–19 cells to evaluate the effect of downstream pathway of PDGF under presence of PD98059 (MAPK inhibitor) or wortmannin (PI3K inhibitor). Similarly, the expression level of integrin α1, α2, ß1 was also evaluated using quantitative real–time PCR and flow cytometry. Results: PDGF treatment up–regulated cell–mediated gel contraction and pretreatment of either PD98059 or wortmannin attenuated the contractile force. Real–time PCR and flow cytometry showed that PDGF up–regulated the expression of integrin α1 and α2. Pretreatment of PD98059 attenuated this up–regulation, however wortmannin did not. Conclusions: PDGF up–regulates ARPE19 cell–mediated gel contraction via both MAPK and PI3K. This biological reaction may be due to up–regulated integrin α1 and α2 expression mediated by MAPK, but not by PI3K. The PI3K pathway may induce contractile activity by other mechanism than integrin.
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