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H. Nishiwaki, N. Yamaike, Y. Hirata, N. Yoshimura, Y. Takaguchi; Photodynamic Therapy With a New Photosensitizer,Fullerodendrimer, Showed Cytotoxity in Human Umbilical Endothelial Cells . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5389.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Fullerodendrimers consist of fullerens and dendrimers. Fullerens such as C60,produce singlet oxygen effectively with light irradiation. Dendrimers are unique macromolecules with highly branched structures and globular shapes. These characteristics of dendrimers will be useful as drug carriers with target cell specificity. In the present study, we investigated activity of fullerodendrimers to inhibit proliferation of human umbilical endothelial cells(HUVECs) with laser irradiation. Methods: We synthesized the fullerodendrimer,C60(G1.5–COOK), by the use of a Diels–Alder reaction of C60 with anthryl dendron. The fullerodendrimer has an excitation wavelength at 685nm. A semiconductor laser(685nm) was used as the light source for exciting the fullerodendrimer. HUVECs (1.0×104cells/ml)were plated onto 96–well cell culture plates. After 24h incubation, the fullerodendrimer at different concentrations (0–10µg/ml) were added to the 120 wells , then 90 wells were irradiated with 10–30J/cm2 using the semiconductor laser and 30 wells were not irradiated. After 48h incubation,cell viability was determined by a modified MTT assay. Results: The combination of the fullerodendrimer and the laser irradiation decreased the cell viability as a function of laser energy and drug conentrations.The fullerodendrimer at 10µg/ml with the laser irradiation of 10J,20J,30J/cm2 reduced the survival rate to 56.3%(P<0.01), 41.5%(P<0.01),29.8%(P<0.01) of HUVECs respectively. In contrast, the fullerodendrimer alone or the laser irradiation alone showed no cyototoxity in HUVECs. Conclusions: The present study suggests that the fullerodendrimer with the laser irradiation inhibits the proliferation of HUVECs.Fullerodendrimers have a potential to be a new photosensitizer for PDT.
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