Purchase this article with an account.
R.N. Khurana, Y.–H. Chang, A. Barnes, G. Fujii, E. de Juan, Jr, M. Humayun; A Novel Method to Oxygenate Intraocular Irrigation Fluids With an In–line Oxygenator . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5512.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Animal studies have demonstrated that oxygen delivered into the vitreous can prolong retinal function during times of ischemia. We describe a novel method to oxygenate intraocular irrigating fluids during vitreoretinal surgeries in–line. Solutions of either Lactated Ringers or Balanced Salt Solution (BSS) were oxygenated with the FE390 Stainless in–line Oxygenation Assembly (Beer, Beer, and More Beer, Concord, CA). After running a 100 ml of solution through the in–line oxygenator, oxygen saturation was measured with a dissolved oxygen meter (Figure 1). A control experiment involving the oxygenator without oxygen perfusion was performed. Paired t–tests were used to compare oxygen saturation changes before and after oxygenation. In comparison to the original solution of BSS, there was an increase in oxygen saturation of 162%±47 (n=40; p<0.05). Without oxygenation perfusion, there was only a 24% ±14 (n=40) increase in oxygen levels in the BSS solution which was significantly lower than the treated BSS solution (p<0.05). In comparison to the original solution of Lactated Ringers, there was an increase in oxygen saturation of 208%±21 (n=40; P<0.05). Without oxygenation perfusion, there was only a 21%±9 (n=40) gain in oxygen saturation in the control group of LR that was statistically lower as well (p<0.05). The in–line oxygenator is an efficient method to oxygenate BSS and LR fluids. It represents a potential efficient and convenient method to oxygenate irrigating solutions for vitreoretinal surgeries.
This PDF is available to Subscribers Only