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Y.–J. Peng, L.–S. Chang, P.–C. Wu, Y.–H. Chen, H.–K. Kuo; Effectiveness of Gas–Compression Vitrectomy and Mechanical Vitrectomy as a Part of Experimental Proliferative Vitreoretinopathy Models in Rabbits . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5524.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the effectiveness of different kinds of experimental proliferative vitreoretinopathy models in rabbits, we designed vitrectomy surgeries in rabbit eyes with either gas compression or vitrector followed by intravitreal injection of cultured homologous RPE cells with or without autologous platelet–rich plasma(PRP). Methods: Four groups of rabbits were studied. Group A (8 eyes) received mechanical vitrectomy followed by intravitreal injection of 5x105 RPE cells and PRP 0.1ml. Group B (4 eyes) received gas compression vitrectomy with 0.2 ml C3F8 followed by intravitreal injection of 5x105 RPE cells and PRP 0.1ml. Group C (3 eyes) received gas compression vitrectomy and intravitreal injection of 1x105 RPE cells and PRP 0.1ml. Group D (3 eyes) received gas compression vitrectomy and intravitreal injection of 1x105 RPE cells only. PVR was observed and graded at day 1,3,7,14,21 and 28. Results: Three eyes of group A were complicated by post–operative dense vitrous hemorrhage and iatrogenic retinal breaks and were excluded. Tractional retinal detachment was successfully induced in all uncomplicated experimental eyes of the four groups by Day 14(Group A,B,C,D–– 5,4,3,3 eyes respectively). Severe intraocular inflammation was noted in all five eyes of group A that made PVR grading difficult. Retinal detail was well accessible in group B,C & D without any surgery–related complication. Gas compression followed by 1x105 RPE cells injection alone (Group D) achieved equal PVR induction rate as the other groups. Conclusions: PVR model in rabbits with gas compression vitrectomy is easily performed with less complication and achieves equal successful rate of tractional retinal detachment induction. Intravitreal injection of RPE cells alone without platelet–rich plasma is effective to induce clinical significant PVR.
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