May 2005
Volume 46, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2005
Increased Nitric Oxide Pathway Metabolites During Retinal Detachment
E.C. LaHey; R.M. H. Diederen; N. Deutz; S.C. Dieudonne; A.T. A. Liem; A. Kijlstra; F. Hendrikse
Author Affiliations & Notes
  • E.C. LaHey
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • R.M. H. Diederen
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • N. Deutz
    Surgery,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • S.C. Dieudonne
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • A.T. A. Liem
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • A. Kijlstra
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • F. Hendrikse
    Ophthalmology,
    Academic Hosp Maastricht, Maastricht, The Netherlands
  • Footnotes
    Commercial Relationships  E.C. LaHey, None; R.M.H. Diederen, None; N. Deutz, None; S.C. Dieudonne, None; A.T.A. Liem, None; A. Kijlstra, None; F. Hendrikse, None.
  • Footnotes
    Support  Algemene Nederlandse Vereniging ter voorkoming van Blindheid
Investigative Ophthalmology & Visual Science May 2005, Vol.46, 5585. doi:
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      E.C. LaHey, R.M. H. Diederen, N. Deutz, S.C. Dieudonne, A.T. A. Liem, A. Kijlstra, F. Hendrikse; Increased Nitric Oxide Pathway Metabolites During Retinal Detachment . Invest. Ophthalmol. Vis. Sci. 2005;46(13):5585.

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Abstract

Abstract: : Purpose: The aim of our study was to examine whether rhegmatogenous retinal detachment (RRD) is associated with increased levels of nitric oxide (NO), citrulline and arginine. Methods: Undiluted vitreous samples were obtained from 151 patients undergoing pars plana vitrectomy. Vitreous concentrations of 2 amino acids, citrulline and arginine were determined by high–pressure liquid chromatography (HPLC). Using the HPLC method we also measured levels of nitrite, the stable product of NO. The study group consisted of 93 patients (93 eyes) with a rhegmatogenous retinal detachment (RRD). The control group consisted of vitreous samples from 25 eyes with an idiopathic macular hole and 24 eyes with an idiopathic epiretinal membrane. Vitreous from eyes with a traction retinal detachment (n=9) due to proliferative diabetic retinopathy (PDR) was also investigated. Results: The mean vitreous concentration of citrulline and arginine was significantly increased in eyes with RRD (9.6±4.3 and 97.2±29.2; respectively) compared to control eyes (7.1±3.2 and 75.9±18.1; respectively) (P= 0.034 and P= 0.002; respectively). Also the mean concentration of nitrite was higher in vitreous of patients (2.24±1.4) compared to controls (2.01±0.72; P=0.058). In addition, a positive correlation was found between vitreous concentration of citrulline and arginine and pre–operative visual acuity (P=0.001 for both amino acids). The mean vitreous concentration of citrulline, arginine and nitrite was significantly elevated in eyes with PDR (25.8±10.3; 130.7±23.7; 2.21±1.53; respectively) compared to control eyes (P=<0.001; P= 0<001; P=0.004, respectively). Conclusions: Increased nitric oxide pathway metabolites were found in eyes with rhegmatogenous retinal detachment. This increase may be related to the retinal hypoxia and NO may play a role in the pathogenesis of photoreceptor damage after retinal detachment.

Keywords: retinal detachment • nitric oxide • vitreous 
© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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