Abstract
Abstract: :
Purpose: To determine the influence of three types of intraocular lenses (IOLs) on human lens epithelial cell behavior and the expression of extracellular matrix proteins produced by these cells on IOLs in vitro. Methods: Human lens epithelial cells (SRA 01/04, provided by Dr. V. N. Reddy) were grown on SA60AT ACRYSOF®, MZ60BD EXPAND® PMMA, and SI–30NB PHACOFLEX® silicone IOLs. Cellular adhesion, migration, proliferation, and apoptotic assays were performed to assess cell behavior. The expression of extracellular matrix proteins was determined by immunoblotting, immunohistochemistry, and metabolic labeling with [3H]–Proline. Results: Cells grown on hydrophobic acrylic lenses had significantly higher proliferation rates in comparison to cells grown on PMMA or silicone IOLs. A confluent monolayer of cells covered the entire anterior surface of the hydrophobic acrylic lenses, but not PMMA or silicone IOLs. More apoptotic cells were present on the PMMA lenses compared to the hydrophobic acrylic lenses. Fibronectin, laminin, and collagens I and IV were produced by cells on all three types of IOLs. Interestingly, there was a decreased production of fibronectin and laminin per cell on the hydrophobic acrylic lens surface compared to the other two types of materials. Cells grown on the hydrophobic acrylic lens also expressed less collagen I compared to cells grown on PMMA or silicone. Conclusions: Lens epithelial cells propagated on the hydrophobic acrylic lenses achieved a confluent monolayer, maintained normal viability and expressed less of some ECM proteins compared to cells grown on other IOL materials. This suggests that hydrophobic acrylic ACRYSOF lenses are more biocompatible than EXPAND PMMA and PHACOFLEX silicone lenses.
Keywords: posterior capsular opacification (PCO) • extracellular matrix • immunohistochemistry