May 2004
Volume 45, Issue 13
ARVO Annual Meeting Abstract  |   May 2004
Innervation of the anterior segment of the eye by secretoneurin
Author Affiliations & Notes
  • E. Schmid
    Department of Ophthalmology & Optometry, University of Innsbruck, Innsbruck, Austria
  • Footnotes
    Commercial Relationships  E. Schmid, None.
  • Footnotes
    Support  P14022–Med from FWF
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 415. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E. Schmid; Innervation of the anterior segment of the eye by secretoneurin . Invest. Ophthalmol. Vis. Sci. 2004;45(13):415.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To evaluate whether secretoneurin (SN) represents a sensory neuropeptide innervating the anterior segment of the eye. Methods: The trigeminal ganglia (TG), the ganglia cervicale superius (GCS) and eye tissues were analyzed for the presence of SN by a highly sensitive radioimmunoassay both in capsaicin pretreated and sympathetically denervated rats. The expression of secretogranin II (SgII) mRNA was studied in the rat TG by in situ hybridization and the processing of SgII by gel filtration chromatography. Then, the concentration of the peptide was determined in human eye tissues and nerve fibers were visualized by immunohistochemistry. Results: There were significant amounts of SN present within the TG and capsaicin pretreatment led to a 57.01% (± 4.29) and 59.07% (±11.86) decrease of the concentration in the TG and the iris/ciliary body complex, respectively. Sympathetic denervation by axotomy failed to lower the concentration on SN in particular eye tissues. The SgII probe labeled numerous cells of various size within the TG and the precursor of the peptide was found to become completely processed to SN. In human eyes, the highest levels of the peptide were found in the choroid and nerve fibers could be visualized predominantly in the iris/ciliary body complex and the choroid. Conclusion: There is unequivocal evidence that SN represents a constituent of capsaicin–sensitive sensory neurons innervating the eye and indicates participation of this peptide in sensory transmission.

Keywords: neuropeptides • anterior segment • anatomy 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.