May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
The effects of glucose on the expression of MMP and TIMP in cultured retinal pigment epithelial cells
Author Affiliations & Notes
  • J. Lee
    Department of Ophthalmology, Inje University/Paik Hospital, Seoul, Republic of Korea
  • J. Kim
    Department of Ophthalmology, Inje University/Paik Hospital, Seoul, Republic of Korea
  • Y. Nah
    Department of Ophthalmology, Inje University/Paik Hospital, Seoul, Republic of Korea
  • H. Cho
    Department of Ophthalmology, Chungang University/Pildong Hospital, Seoul, Republic of Korea
  • H. An
    Department of Ophthalmology, Inje University/Paik Hospital, Seoul, Republic of Korea
  • Y. Kim
    Department of Ophthalmology, Inje University/Paik Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  J. Lee, None; J. Kim, None; Y. Nah, None; H. Cho, None; H. An, None; Y. Kim, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 445. doi:https://doi.org/
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    • Get Citation

      J. Lee, J. Kim, Y. Nah, H. Cho, H. An, Y. Kim; The effects of glucose on the expression of MMP and TIMP in cultured retinal pigment epithelial cells . Invest. Ophthalmol. Vis. Sci. 2004;45(13):445. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: This study evaluated the effects of glucose on proliferation, migration, and proteinase expressions in human retinal pigment epithelial cells to discover the cause of diabetic retinal complications. Methods: Human retinal pigment epithelial cells (CRL 2302) were cultured in media containing 5.5mM, 11.0mM, and 16.5mM D–glucose to simulate diabetic condition. The present study performed a MTT analysis for the proliferation assay and counted a number of cells for the migration assay. In addition, the study conducted western blotting for the expression of protein of matrix metalloproteinase(MMP)–2, –9, tissue inhibitor of matrix metalloproteinase(TIMP)–1, –2 , and RT–PCR for the expression of mRNA of MMP–2,9, and TIMP–1, –2. Results: The findings of the MTT and cell–count indicated that raising concentration of glucose resulted in a significant decrease in cellular proliferation and migration. The results of the western blotting analysis illuminated that raising concentration of glucose significantly increased the expression of MMP–2 and –9 but significantly decreased the expression of TIMP–1 and –2. Moreover, the RT–PCR results indicated significant increases in the expression of mRNA of MMP–2 and –9 as well as in the expressions of mRNA of TIMP–1 and –2 by raising glucose concentration. Conclusions: Higher levels of glucose showed significant effect on the cellular proliferation, migration, and expression of MMP and TIMP in human retinal pigment epithelial cells. This study provides fundamentals in later work on the mechanism of retinal complication in diabetic patients.

Keywords: retinal pigment epithelium • enzymes/enzyme inhibitors • diabetes 
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