May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Subclinical conjunctival changes in patients with corneal allograft
Author Affiliations & Notes
  • E. Lala–Gitteau
    Ophthalmology,
    Hopital Bretonneau, University François Rabelais, Tours, France
  • F. Brignole
    Lab. Toxicology, Faculty Biological and Pharmaceutical Sciences University René Descartes, Paris V, Paris, France
  • J. Rateau
    Ophthalmology,
    Hopital Bretonneau, University François Rabelais, Tours, France
  • C. Baudouin
    Ophthalmology, CHNO des Quinze–Vingts, EA 3123, University Pierre & Marie Curie, Paris VI, Paris, France
  • J.–M. Warnet
    Lab. Toxicology, Faculty Biological and Pharmaceutical Sciences University René Descartes, Paris V, Paris, France
  • Y. Lebranchu
    Immunology,
    Hopital Bretonneau, University François Rabelais, Tours, France
  • P.–J. Pisella
    Ophthalmology,
    Hopital Bretonneau, University François Rabelais, Tours, France
  • Footnotes
    Commercial Relationships  E. Lala–Gitteau, None; F. Brignole, None; J. Rateau, None; C. Baudouin, None; J. Warnet, None; Y. Lebranchu, None; P. Pisella, None.
  • Footnotes
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Investigative Ophthalmology & Visual Science May 2004, Vol.45, 609. doi:
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    • Get Citation

      E. Lala–Gitteau, F. Brignole, J. Rateau, C. Baudouin, J.–M. Warnet, Y. Lebranchu, P.–J. Pisella; Subclinical conjunctival changes in patients with corneal allograft . Invest. Ophthalmol. Vis. Sci. 2004;45(13):609.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:To investigate using impression cytology specimens (IC) the expression of inflammatory and immune–related markers by conjunctival epithelial cells taken from corneal allografted patients. Methods:Seventy eyes (from 70 patients) with corneal allograft, 18 eyes (from 10 patients) with inflammatory ocular surface, and 26 eyes from 13 healthy control subjects were included in a non–randomized, prospective and comparative study. IC were collected after clinical examination and analyzed by flow cytometry, using monoclonal antibodies directed against CD45, HLA DR, CD1a and Langerin. The percentage of positive cells and levels of fluorescence were calculated and compared between each group. A further morphological study with confocal and electronic microscopy was also realized. Results:A significant increase of CD45 and HLA DR expression, positively correlated each other, was found in both pathologic groups as compared to the control group. CD1a and Langerin expression, also positively correlated each other, were significantly increased in corneal allografted eyes as compared to healthy or non–specific inflammatory eyes. While CD45 and HLA DR expression decreased in time after penetrating keratoplasty, the expression of both CD1a and Langerin increased in the same post–operative period. Conclusion:Corneal allograft seems to be associated with conjunctival subclinical changes. Indeed, conjunctival cells from patients with corneal allograft overexpress dendritic cells–related markers. These data support the use of immunomodulatory topical drugs in this indication and show a potential interest of IC in the immunologic follow–up of corneal allograft.

Keywords: conjunctiva • flow cytometry • transplantation 
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