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K. Sasanuma, S. Yoshizaki, S. Nakamura, F. Saito; Suppression of Tear Fluid Proinflammatory Cytokines by D–3–Hydroxybutyrate(HBA) in a Rat Dry Eye Model . Invest. Ophthalmol. Vis. Sci. 2004;45(13):68.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: We previously demonstrated that topically applied HBA significantly prevented corneal epithelial cell degeneration through suppression of apoptosis under dry eye conditions in rat (IOVS 2003 44: 4682–4688). This study investigated the obvious relations between the protective effect of HBA and apoptotic proinflammatory cytokine in tear fluid. Methods: Female 8–week–old Sprague–Dawley rats (n=8) were used in this study. After acclimatization, the rats were placed under controlled standard conditions (room temperature 23±2°C, relative humidity 60±10%) for 3 hours. Then, the rats were transferred to a dry conditioned room (23±2°C, relative humidity 28±2%, constant air flow 2–4m/s) and were maintained for 5 hours, just after the central region of the corneal epithelium (0.4 mm2) was scraped. Ten microlitres of 80mM HBA or phosphate–buffered saline (PBS) were topically applied to the rat eye every hour. At the end of treatment, tear fluid was collected from each eye using a cotton thread. Samples were extracted with PBS and the concentrations of IL–1α were determined using ELISA. Results: In PBS–treated eyes, the tear fluid concentration of IL–1α was drastically increased when rats were transferred from a standard condition to a dry condition (5.46±0.91 to 10.98±2.05 ng/mL, p < 0.05). In HBA–treated eyes, the increase in IL–1α was moderate (4.86±0.63 to 8.95±2.11 ng/mL, N.S.). There was no apparent change in IL–1α concentrations observed during the standard condition with corneal epithelial wounding or the dry condition without wounding. Conclusions: These results indicate that HBA could suppress the increase in tear fluid proinflammatory cytokines induced by dry eye condition.
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