May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Evaluation of –Glutamyltranspeptidase Activity Levels in Diabetic and Non–DiabeticTears Using Schirmer Test Strips.
J.P. Ganley; J. Gosslee; V. Murray; M. Sakhalkar; J. DellaCroce; V. Chen–Espinoza; J. Hinrichsen; J. Wood; T.B. Redens; M.P. Langford
Author Affiliations & Notes
  • J.P. Ganley
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • J. Gosslee
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • V. Murray
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • M. Sakhalkar
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • J. DellaCroce
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • V. Chen–Espinoza
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • J. Hinrichsen
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • J. Wood
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • T.B. Redens
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • M.P. Langford
    Ophthalmology, LSU Health Sciences Center, Shreveport, LA
  • Footnotes
    Commercial Relationships  J.P. Ganley, None; J. Gosslee, None; V. Murray, None; M. Sakhalkar, None; J. DellaCroce, None; V. Chen–Espinoza, None; J. Hinrichsen, None; J. Wood, None; T.B. Redens, None; M.P. Langford, None.
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 96. doi:
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      J.P. Ganley, J. Gosslee, V. Murray, M. Sakhalkar, J. DellaCroce, V. Chen–Espinoza, J. Hinrichsen, J. Wood, T.B. Redens, M.P. Langford; Evaluation of –Glutamyltranspeptidase Activity Levels in Diabetic and Non–DiabeticTears Using Schirmer Test Strips. . Invest. Ophthalmol. Vis. Sci. 2004;45(13):96.

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Abstract

Abstract: : Purpose: Investigate γ–glutamyltranspeptidase (GGT; extracellular enzyme that catalyses the conversion of glutamine and glutathione to glutamate) activity in tears of persons with glaucoma, diabetic and non–diabetic disease. Methods: Five minute Schirmer tear (STS) tests were performed on 190 consenting individuals seen at the LSUHSC Eye Clinic, the amount of tear/STS, age, sex, and significant past medical history for ocular and systemic disease was recorded. The GGT (production of γ–glutamyl–p–nitroanaline) and protein/STS were determined by standard assays and the amount of GGT was calculated. Results: The mean age of the study group was 54.3 ±14.8 years and females outnumbered males 3:1. The mean tear volume collected/5 min was 21.1±3.9 mm/STS/eye. The mean GGT activity/STS was 6.75±8.8 nM/hr. The amount of GGT activity/STS was positively correlated with mm tear/STS (Corr. coef. = 0.449; slope 1.823), but tear protein/STS was not related to mm tear/STS or GGT/STS. Tear production was not affected by age or sex, but was reduced by topical anesthetic. Comparative analyses of the GGT in STS tip (contacts conjunctival tissue) and shaft (tear film), indicated that the STS tip GGT levels in non–diabetic patient control eyes (5.52 ±3.54 nM/hr), non–diabetic glaucomatous (5.36 ±3.23 nM/hr) and non–insulin–dependent diabetic (NIDDM) eyes (6.13 ±4.54 nM/hr) were higher than in insulin–dependent diabetic (IDDM) eyes (4.2 ±3.1 nM/hr; p’s< 0.01). The STS shaft GGT levels in non–diabetic control, glaucomatous and NIDDM eyes (3.75 ±2.82 nM/hr, 4.21 ± 2.61 nM/hr, and 4.44 ±4.1 nM/hr, respectively) were not significantly higher than the STS shaft GGT levels in IDDM eyes (2.94 ±2.3 nM/hr; p’s> 0.09). Conclusions: The low levels of GGT activity in conjunctival tissue adherent to STS tips from eyes of persons with a medical history of IDDM parallels the reduced serum and retinal GGT levels in these individuals. The reduction of GGT could affect glutamine, glutathione, glutamate, melatonin and leukotriene metabolism in the conjunctival associated tissues. The reduced GGT may be due to a cellular defect associated with IDDM or may be an indicator of diabetic control by insulin therapy.

Keywords: conjunctiva • pathology: human • diabetes 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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