May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Proteomics analysis for the screening of retinal development factors
S. Tanimoto; T. Kanamoto; T. Yokoyama; N. Noma; H. Aoyama; H.K. Mishima
Author Affiliations & Notes
  • S. Tanimoto
    Ophthalmology and Visual Science,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • T. Kanamoto
    Ophthalmology and Visual Science,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • T. Yokoyama
    Ophthalmology and Visual Science,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • N. Noma
    Ophthalmology and Visual Science,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • H. Aoyama
    Anatomy and Developmental Biology,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • H.K. Mishima
    Ophthalmology and Visual Science,
    Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
  • Footnotes
    Commercial Relationships  S. Tanimoto, None; T. Kanamoto, None; T. Yokoyama, None; N. Noma, None; H. Aoyama, None; H.K. Mishima, None.
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 673. doi:
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      S. Tanimoto, T. Kanamoto, T. Yokoyama, N. Noma, H. Aoyama, H.K. Mishima; Proteomics analysis for the screening of retinal development factors . Invest. Ophthalmol. Vis. Sci. 2004;45(13):673.

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Abstract

Abstract: : Purpose: To analyze the mechanism of retinal development. Methods: Two protein lysate samples, that embryonic day 7 and 11, were prepared from chick embryo retina. Total retina lysates were electrophoresed on two–dimensional SDS page gels, and isolated protein spots on gels were detected by silver staining. The change of all spots expressions were differentially displayed, dependent on embryonic day, by the software ( PD–Quest : BioRad). The target spots, protein expressions were dramatically changed, were selected, and the proteins of targets were identified by mass –spectrometry ( Q–STAR : Applied Biosystems ). Moreover, the functional analysis of target proteins was tried out, using three dimensional tissue culture of chick embryo retina. Results: Many target proteins, as suspected retinal differentiation factors, were detected, and we made clustering analysis of target proteins. Conclusions: Proteomics analysis of chick embryo retina is succeeded, and we suggest that the target proteins may act on retinal development.

Keywords: proteomics • retina • retinal culture 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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