May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Upregulation of Antiangiogenic Genes by Transpupillary Thermotherapy
Author Affiliations & Notes
  • Y. Ito
    Ophthalmology, Saitama Medical School, Saitama, Japan
  • S. Yoneya
    Ophthalmology, Saitama Medical School, Saitama, Japan
  • H. Takita
    Ophthalmology, Saitama Medical School, Saitama, Japan
  • M. Ito
    Ophthalmology, Saitama Medical School, Saitama, Japan
  • K. Mori
    Ophthalmology, Saitama Medical School, Saitama, Japan
  • Footnotes
    Commercial Relationships  Y. Ito, None; S. Yoneya, None; H. Takita, None; M. Ito, None; K. Mori, None.
  • Footnotes
    Support  Grant–in–Aid for Scientific Research, Japan Society for the Promotion of Science, 13771041
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 706. doi:
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    • Get Citation

      Y. Ito, S. Yoneya, H. Takita, M. Ito, K. Mori; Upregulation of Antiangiogenic Genes by Transpupillary Thermotherapy . Invest. Ophthalmol. Vis. Sci. 2004;45(13):706.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To describe a possible molecular mechanism of closure of choroidal neovascularization induced by transpupillary thermotherapy (TTT). Methods: The five posterior fundi of five Brown Norway rats were exposed to an 810–nm diode laser around an optic disc, and the contralateral control eyes received no treatment. Six TTT lesions per eye were placed at the following laser–power setting; at 50mW for 60 seconds 3mm in diameter. Eyes were enucleated 2 hours after the treatment and mRNA was extracted. Samples were processed for cDNA microarray analysis to search genes with increased expression induced by TTT. Hybridization signals were visualized with cyanine (Cy)–5 and Cy–3, and analyses were duplicated by two–color dye swapping method. Semi–quantitative reverse transcription PCR was performed to validate the microarray results. Results: Laser–induced lesions were not visible by funduscopy 2 hours after TTT, which indicated the current laser–power setting was sub–threshold. Of the 14815 cDNA elements on the array 12 genes were upregulated in TTT treated eyes, such as MIC–1, metallothionein–1, metallothionein–2, JE/MCP–1, interleukin 1–beta, c–jun, asparagine synthetase, sestrin p53 regulated PA26–T3 nuclear protein, thrombospondin–1, NAD–dependent methylenetetrahydrofolate, fibrinogen–like protein, endothelial cell activated protein C receptor. Among these genes, RT–PCR revealed that thrombospondin–1, JE/MCP–1, NAD–dependent methylenetetrahydrofolate, asparagine synthetase and MIC–1 were increased their expression in TTT eyes. Conclusions:The current study clearly demonstrated that TTT induces upregulation of antiangiogenic genes, such as thrombospondin–1. This phenomenon is a possible explanation for the molecular mechanisms of vascular occlusion induced by TTT.

Keywords: gene/expression • laser • choroid: neovascularization 
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