May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Subtractive hybridization reveals differentially expressed genes in retinas of rats raised in dim and bright cyclic light
Author Affiliations & Notes
  • R.E. Anderson
    Ophthalmology, Cell Biology,
    Univ of Oklahoma Hlt Sci Ctr, Oklahoma City, OK
    Dean A. McGee Eye Institute, Oklahoma City, OK
  • Q. Zhang
    Cell Biology,
    Univ of Oklahoma Hlt Sci Ctr, Oklahoma City, OK
    Dean A. McGee Eye Institute, Oklahoma City, OK
  • Footnotes
    Commercial Relationships  R.E. Anderson, None; Q. Zhang, None.
  • Footnotes
    Support  NIH grants EY00871, EY04149, EY12920, and RR17730; RPB; FFB
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 716. doi:
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      R.E. Anderson, Q. Zhang; Subtractive hybridization reveals differentially expressed genes in retinas of rats raised in dim and bright cyclic light . Invest. Ophthalmol. Vis. Sci. 2004;45(13):716.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To search for candidate genes that provide neuroprotection against light–induced apoptosis in retinas of rats raised in bright cyclic light. Methods: Rats (Sprague–Dawley) were born and raised in dim (5 lux) or bright (400 lux). Retinas from the animals of 7 weeks of age were dissected and used for RNA extraction and subtractive hybridization. We used a cDNA subtractive hybridization technique to seek differentially expressed genes in the retinas of rats raised in bright cyclic light. The clones from the subtraction were characterized by DNA sequencing, bioinformatics, and northern blot analysis. Results:We identified 14 clones up–regulated by forward subtraction and 19 clones down–expressed in bright cyclic light by reverse subtraction. Results of DNA sequencing and bioinformatics revealed that the clones include the genes of metabolism, phototransduction, enzymes, and unknown function proteins. Conclusions:Subtraction technique is an approach for searching for differentially expressed gene in the retina of rat raised by bight cyclic light. Currently we are evaluating the expression of the identified genes and their potential roles in rat retinal neuroprotection.

Keywords: neuroprotection • gene/expression • retinal degenerations: cell biology 
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