May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Effect of tauroursodeoxycholic acid on retinal degeneration in rd10 mice
Author Affiliations & Notes
  • J.H. Boatright
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
  • A.G. Moring
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
  • C.E. McElroy
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
    Rehabilitative Medicine Research and Development, VA Medical Center, Atlanta, GA
  • V.T. Do
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
  • M.J. Phillips
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
    Rehabilitative Medicine Research and Development, VA Medical Center, Atlanta, GA
  • J.M. Nickerson
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
  • M.T. Pardue
    Ophthalmology, Emory Univ School of Medicine, Atlanta, GA
    Rehabilitative Medicine Research and Development, VA Medical Center, Atlanta, GA
  • Footnotes
    Commercial Relationships  J.H. Boatright, None; A.G. Moring, None; C.E. McElroy, None; V.T. Do, None; M.J. Phillips, None; J.M. Nickerson, None; M.T. Pardue, None.
  • Footnotes
    Support  NIH Grant R01 EY12514, R03 EY13986, P30 EY06360, P30 AT000609, FFB, RPB
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 720. doi:
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      J.H. Boatright, A.G. Moring, C.E. McElroy, V.T. Do, M.J. Phillips, J.M. Nickerson, M.T. Pardue; Effect of tauroursodeoxycholic acid on retinal degeneration in rd10 mice . Invest. Ophthalmol. Vis. Sci. 2004;45(13):720.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Tauroursodeoxycholic acid (TUDCA) is a naturally–occurring bile acid that is neuroprotective in models of neurological disease. We tested whether TUDCA was similarly protective in the rd10 mouse, an animal model of retinal degeneration. Methods: Mice were injected subcutaneously with TUDCA or vehicle (NaHCO3) during development. At postnatal day 18 (PN18), electroretinograms (ERGs) were measured and mice were killed and their eyes harvested and fixed. Eyes were sectioned, sections stained (H & E or toluidine blue), and retina cell layers morphometrically analyzed by light microscopy. Consecutive sections were subjected to modified TUNEL. Results: Retinas of TUDCA–treated mice had thicker outer nuclear layers, more photoreceptor cells, more fully–developed photoreceptor outer segments, and fewer TUNEL–positive cells compared to those treated with vehicle. ERG a–wave and b–wave amplitudes were greater in mice treated with TUDCA compared to those treated with vehicle. ERG, morphometric, and TUNEL differences were statistically significant. No deleterious effects were observed with TUDCA or vehicle injections. Conclusions: TUDCA treatment partially prevented photoreceptor degeneration and loss of visual function in rd10 mice at PN18, possibly by suppressing apoptosis.

Keywords: apoptosis/cell death • pharmacology • photoreceptors 
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