May 2004
Volume 45, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2004
Pinoline protects the retina from lipid peroxidation
A.W. Siu; B.B. Tam; E.Y. Lee; A.F. Lam
Author Affiliations & Notes
  • A.W. Siu
    Optometry/Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • B.B. Tam
    Optometry/Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • E.Y. Lee
    Optometry/Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • A.F. Lam
    Optometry/Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • Footnotes
    Commercial Relationships  A.W. Siu, None; B.B. Tam, None; E.Y. Lee, None; A.F. Lam, None.
  • Footnotes
    Support  PolyU Grant G–T485
Investigative Ophthalmology & Visual Science May 2004, Vol.45, 734. doi:
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      A.W. Siu, B.B. Tam, E.Y. Lee, A.F. Lam; Pinoline protects the retina from lipid peroxidation . Invest. Ophthalmol. Vis. Sci. 2004;45(13):734.

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Abstract

Abstract: : Purpose: Pinoline is a pineal indoleamine naturally found in the retina. This study compared the effects of pinoline and vitamin E on the copper(I)–induced retinal lipid peroxidation (LPO). Methods: Procine retinal homogenates were mixed with 120µM copper(I) solution. The mixtures were co–incubated with various concentrations of pinoline or trolox (water–soluble vitamin E analogue) at 37°C for 60min. The amounts of malondialdehyde (MDA) and protein were then assayed to quantify the LPO. Results: Copper (I) ions significantly increased the MDA concentration in the retinal homogenates (p<0.0007). Both pinoline and trolox significantly suppressed MDA in a dose–dependent manner (p<0.0001) and their effects were significantly different (p=0.004). The concentrations that inhibited 50% of LPO were 0.24 mM and 0.68 mM for pinoline and trolox respectively. Conclusions: Pinoline demonstrated an anti–LPO potency of 2.8 times compared with trolox. The results support an anti–oxidative role of pinoline in the retina.

Keywords: retina • oxidation/oxidative or free radical damage • pathobiology 
© 2004, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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