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E.T. Brown, Y. Umino, E. Solessio, T. Loi, R. Quinn, R. Barlow; Anesthesia Affects Mouse ERG and Blood Glucose . Invest. Ophthalmol. Vis. Sci. 2004;45(13):742.
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Purpose: Investigate the effect of common anesthetics on both the ERG and blood glucose of mice. Methods: Fed and fasted C57/BL6J mice were injected I.P. with one of four anesthetics (see below). Under dark–adapted conditions their pupils were dilated with 1% tropicamide and corneas were moistened with methylcellulose (Moisture Eyes, Bausch Lomb). A unipolar Burian Allen mouse ERG electrode was placed on the cornea with reference in the mouth. ERGs were recorded (100gain, 0.3–1000Hz) in response to 10–ms flashes delivered under computer control (P–Clamp, Axon Instruments) from a cluster of 520nm LEDs placed 1cm from the cornea (Maximum intensity of 230µW /cm2). The animal was maintained near 380C with a heating pad. Using a OneTouch Ultra glucose meter blood glucose (BG) levels were measured from tail blood before anesthesia, 15min and 60min after injection of anesthetic. Results:The effects of four anesthetics and saline control after 60min are:
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